TY - JOUR
T1 - Antibiotic susceptibility testing of Staphylococcus aureus using the Biolog OmniLog® system, a metabolic phenotyping assay
AU - Vaillant, James J.
AU - Cunningham, Scott A.
AU - Patel, Robin
N1 - Funding Information:
This work was supported by the Mayo Clinic Division of Public Health, Infectious Diseases, and Occupational Medicine . The funding source did not participate in study design, data collection, analysis, or interpretation, writing of the report, or the decision to submit the article for publication.
Funding Information:
RP reports grants from ContraFect, TenNor Therapeutics Limited, and BioFire. RP is a consultant to Curetis, PathoQuest, Selux Diagnostics, 1928 Diagnostics, PhAST, Torus Biosystems, Day Zero Diagnostics, Mammoth Biosciences, and Qvella; monies are paid to Mayo Clinic. Mayo Clinic and RP have a relationship with Pathogenomix. RP has research supported by Adaptive Phage Therapeutics. Mayo Clinic has a royalty-bearing know-how agreement and equity in Adaptive Phage Therapeutics. RP is also a consultant to Netflix, Abbott Laboratories, and CARB-X. In addition, RP has a patent on Bordetella pertussis/parapertussis PCR issued, a patent on a device/method for sonication with royalties paid by Samsung to Mayo Clinic, and a patent on an anti-biofilm substance issued. RP receives honoraria from the NBME, Up-to-Date and the Infectious Diseases Board Review Course.
Publisher Copyright:
© 2022 Mayo Clinic
PY - 2022/10
Y1 - 2022/10
N2 - Use of the Biolog OmniLog® phenotyping system for antibiotic susceptibility testing (AST) was evaluated using 51 clinical isolates of Staphylococcus aureus. MIC testing by broth microdilution was compared to results generated using the OmniLog® system for oxacillin, daptomycin, vancomycin, gentamicin, linezolid, and tetracycline. There was >90% essential and categorical agreement between methods for all antibiotics, except gentamicin, which had 83.6% essential agreement, although very major errors occurred with linezolid (n = 3) and daptomycin (n = 1). Precision was satisfactory, with 5 triplicate measurements in agreement. A quantitative threshold allowed automated interpretation of MICs yielding results comparable to manual interpretation; oxacillin, gentamicin, and tetracycline resistance could be identified at a median of 7.13, 5.25, and 7.25 hours, respectively. Limitations include the small number of isolates, and especially resistant isolates tested, and the focus on a single species. Overall, the OmniLog® system was a precise method for AST of S. aureus, although accuracy was imperfect.
AB - Use of the Biolog OmniLog® phenotyping system for antibiotic susceptibility testing (AST) was evaluated using 51 clinical isolates of Staphylococcus aureus. MIC testing by broth microdilution was compared to results generated using the OmniLog® system for oxacillin, daptomycin, vancomycin, gentamicin, linezolid, and tetracycline. There was >90% essential and categorical agreement between methods for all antibiotics, except gentamicin, which had 83.6% essential agreement, although very major errors occurred with linezolid (n = 3) and daptomycin (n = 1). Precision was satisfactory, with 5 triplicate measurements in agreement. A quantitative threshold allowed automated interpretation of MICs yielding results comparable to manual interpretation; oxacillin, gentamicin, and tetracycline resistance could be identified at a median of 7.13, 5.25, and 7.25 hours, respectively. Limitations include the small number of isolates, and especially resistant isolates tested, and the focus on a single species. Overall, the OmniLog® system was a precise method for AST of S. aureus, although accuracy was imperfect.
KW - OmniLog
KW - Staphylococcus aureus
KW - antimicrobial susceptibility testing
KW - broth microdilution
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U2 - 10.1016/j.diagmicrobio.2022.115759
DO - 10.1016/j.diagmicrobio.2022.115759
M3 - Article
C2 - 35872370
AN - SCOPUS:85134631252
SN - 0732-8893
VL - 104
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 2
M1 - 115759
ER -