Anti-oxLDL antibody isotype levels, as potential markers for progressive atherosclerosis in APOE-/- and APOE-/-CD40L-/- mice

M. L.F. Smook, M. Van Leeuwen, P. Heeringa, J. G.M.C. Damoiseaux, R. Theunissen, M. J.A.P. Daemen, E. Lutgens, J. W. Cohen Tervaert

Research output: Contribution to journalArticlepeer-review


In humans and animal models of atherosclerosis, antibodies against oxidized LDL have been associated with atherosclerotic lesion development. It has been suggested that IgM anti-oxLDL antibodies are anti-atherogenic, whereas IgG anti-oxLDL antibodies are pro-atherogenic. In this study, we examined the relation between IgM and IgG antibody levels and atherosclerosis severity in APOE-/-CD40L-/- mice, which are deficient for IgG and develop moderate advanced atherosclerosis, and compared results with mice developing severe (APOE-/-) or no atherosclerosis (C57Bl/6). Mice were followed in time for anti-oxLDL antibodies while on high-fat diet or normal chow. Anti-oxLDL antibody levels were determined by ELISA. Results revealed that 24-week-old APOE-/-CD40L-/- mice had enhanced IgM anti-oxLDL antibody levels when compared with wild-type mice, but similar levels to those of APOE-/- mice. As expected, IgG anti-oxLDL antibody levels were almost absent in APOE-/-CD40L-/- mice. The transition from early to advanced lesions in APOE-/- mice was reflected by elevated IgM anti-oxLDL antibody levels. IgM anti-oxLDL levels did not further increase during progression to more advanced lesions. No relation was found between IgG anti-oxLDL levels and atherosclerosis severity. In conclusion, the severity of advanced atherosclerosis in mice is not reflected by IgM and/or IgG anti-oxLDL antibody levels. Furthermore, less advanced atherosclerotic lesion development in APOE-/-CD40L-/- mice does not seem to be the result of higher levels of protective IgM anti-oxLDL antibodies. Therefore, our study does not support the idea that the previously observed inconsistency in the relation between anti-oxLDL and atherosclerosis severity is due to differences in antibody isotypes.

Original languageEnglish (US)
Pages (from-to)264-269
Number of pages6
JournalClinical and Experimental Immunology
Issue number2
StatePublished - Nov 2008


  • Anti-oxLDL antibodies
  • Antibody isotypes
  • Auto-antibodies
  • Mouse

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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