TY - JOUR
T1 - Analysis of p53 mutations in human gliomas by RNA single-strand conformational polymorphism
AU - Cheng, Theresa M.
AU - Ganju, Vinod
AU - Ritland, Steve R.
AU - Sarkar, Gobinda
AU - Jenkins, Robert B.
N1 - Funding Information:
We thank the following for their assistance: Paul Stalboerger for preparation of the tissue, Michael Strausbauch and Kevin Battaile for sequencing advice, and Dr. Gerard Vockley's laboratory for their generosity in allowing us the use of equipment. This work was supported by National Institutes of Health Grant CA 50905.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - This chapter examines the leukocytes and tumor tissue of patients with brain tumors for p53 mutations. Because most mutations that occur in the p53 gene are single base pair mutations, a technique sensitive for the detection of such alteration using RNA single-strand conformational polymorphisms (rSSCP) has been developed. The determination of the genetic cause(s) and pathway(s) of oncogenesis for human cancers is important so that effective diagnosis and treatment modalities may be developed. These pathways are thought to vary from one tumor type to another, although the components are often the same negatively and positively acting gene product cell-cycle regulators. An important negative regulator of tumorigenesis is the p53 protein. The inactivation of the gene by mutation or of the p53 protein itself by oncogene products of tumor viruses is important in several tumors. Germline p53 mutations in patients from pedigree affected with Li-Fraumeni syndrome have also been shown to predispose carriers to a higher incidence of initial primary and second primary cancers, such as brain tumors, sarcomas, leukemias, breast carcinomas, and other neoplasms. Thus, the detection of somatic or germline mutations are important influences in the treatment and prognosis of these patients.
AB - This chapter examines the leukocytes and tumor tissue of patients with brain tumors for p53 mutations. Because most mutations that occur in the p53 gene are single base pair mutations, a technique sensitive for the detection of such alteration using RNA single-strand conformational polymorphisms (rSSCP) has been developed. The determination of the genetic cause(s) and pathway(s) of oncogenesis for human cancers is important so that effective diagnosis and treatment modalities may be developed. These pathways are thought to vary from one tumor type to another, although the components are often the same negatively and positively acting gene product cell-cycle regulators. An important negative regulator of tumorigenesis is the p53 protein. The inactivation of the gene by mutation or of the p53 protein itself by oncogene products of tumor viruses is important in several tumors. Germline p53 mutations in patients from pedigree affected with Li-Fraumeni syndrome have also been shown to predispose carriers to a higher incidence of initial primary and second primary cancers, such as brain tumors, sarcomas, leukemias, breast carcinomas, and other neoplasms. Thus, the detection of somatic or germline mutations are important influences in the treatment and prognosis of these patients.
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U2 - 10.1016/S1043-9471(06)80092-3
DO - 10.1016/S1043-9471(06)80092-3
M3 - Article
AN - SCOPUS:77957095664
SN - 1043-9471
VL - 26
SP - 210
EP - 224
JO - Methods in Neurosciences
JF - Methods in Neurosciences
IS - C
ER -