Analysis of EGF receptor amplicons reveals amplification of multiple expressed sequences

Xiao Yang Wang; David I. Smith; Lori Frederick; C. David James

doi:10.1038/sj.onc.1201476

Analysis of EGF receptor amplicons reveals amplification of multiple expressed sequences

Xiao Yang Wang, David I. Smith, Lori Frederick, C. David James

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

In this study me have examined the epidermal growth factor receptor gene (EGFR) region for the amplification of novel expressed sequences in 28 glioblastoma tumors and two cell lines with EGFR gene amplification. Southern analysis with expressed sequence tag (EST) probes revealed instances of amplification for 11 of 13 loci investigated. Northern blot analysis with the same probes indicated that three ESTs identified transcripts that are over-expressed in cell lines having corresponding sequence amplification. The determination of increased dosage and transcript levels for multiple expressed sequences raises the possibility that there are genes in addition to EGFR whose elevated expression contribute to the biologic behavior of tumors with amplification of this region.

Original language	English (US)
Pages (from-to)	191-195
Number of pages	5
Journal	Oncogene
Volume	16
Issue number	2
DOIs	https://doi.org/10.1038/sj.onc.1201476
State	Published - Jan 15 1998

Keywords

Amplicon
EGFR
EST
Glioblastoma

ASJC Scopus subject areas

Molecular Biology
Genetics
Cancer Research

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10.1038/sj.onc.1201476

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title = "Analysis of EGF receptor amplicons reveals amplification of multiple expressed sequences",

abstract = "In this study me have examined the epidermal growth factor receptor gene (EGFR) region for the amplification of novel expressed sequences in 28 glioblastoma tumors and two cell lines with EGFR gene amplification. Southern analysis with expressed sequence tag (EST) probes revealed instances of amplification for 11 of 13 loci investigated. Northern blot analysis with the same probes indicated that three ESTs identified transcripts that are over-expressed in cell lines having corresponding sequence amplification. The determination of increased dosage and transcript levels for multiple expressed sequences raises the possibility that there are genes in addition to EGFR whose elevated expression contribute to the biologic behavior of tumors with amplification of this region.",

keywords = "Amplicon, EGFR, EST, Glioblastoma",

author = "Wang, {Xiao Yang} and Smith, {David I.} and Lori Frederick and James, {C. David}",

note = "Funding Information: This work was supported by grants from the National Cancer Institute: CA-55728 (CDJ) and CA-48031 (DIS).",

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T1 - Analysis of EGF receptor amplicons reveals amplification of multiple expressed sequences

AU - Wang, Xiao Yang

AU - Smith, David I.

AU - Frederick, Lori

AU - James, C. David

N1 - Funding Information: This work was supported by grants from the National Cancer Institute: CA-55728 (CDJ) and CA-48031 (DIS).

PY - 1998/1/15

Y1 - 1998/1/15

N2 - In this study me have examined the epidermal growth factor receptor gene (EGFR) region for the amplification of novel expressed sequences in 28 glioblastoma tumors and two cell lines with EGFR gene amplification. Southern analysis with expressed sequence tag (EST) probes revealed instances of amplification for 11 of 13 loci investigated. Northern blot analysis with the same probes indicated that three ESTs identified transcripts that are over-expressed in cell lines having corresponding sequence amplification. The determination of increased dosage and transcript levels for multiple expressed sequences raises the possibility that there are genes in addition to EGFR whose elevated expression contribute to the biologic behavior of tumors with amplification of this region.

AB - In this study me have examined the epidermal growth factor receptor gene (EGFR) region for the amplification of novel expressed sequences in 28 glioblastoma tumors and two cell lines with EGFR gene amplification. Southern analysis with expressed sequence tag (EST) probes revealed instances of amplification for 11 of 13 loci investigated. Northern blot analysis with the same probes indicated that three ESTs identified transcripts that are over-expressed in cell lines having corresponding sequence amplification. The determination of increased dosage and transcript levels for multiple expressed sequences raises the possibility that there are genes in addition to EGFR whose elevated expression contribute to the biologic behavior of tumors with amplification of this region.

KW - Amplicon

KW - EGFR

KW - EST

KW - Glioblastoma

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Analysis of EGF receptor amplicons reveals amplification of multiple expressed sequences

Abstract

Keywords

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