Analysis of antiviral drug properties of thymidine kinase of herpes B virus using recombinant herpes simplex virus 1

Phu Hoang Anh Nguyen; Shuetsu Fukushi; Souichi Yamada; Shizuko Harada; Tomoki Yoshikawa; Hitomi Kinoshita; Madoka Kawahara; Takuma Ogawa; Hideki Ebihara; Meng Ling Moi; Masayuki Saijo

doi:10.1128/spectrum.03091-23

Analysis of antiviral drug properties of thymidine kinase of herpes B virus using recombinant herpes simplex virus 1

Phu Hoang Anh Nguyen, Shuetsu Fukushi, Souichi Yamada, Shizuko Harada, Tomoki Yoshikawa, Hitomi Kinoshita, Madoka Kawahara, Takuma Ogawa, Hideki Ebihara, Meng Ling Moi, Masayuki Saijo

Molecular Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

IMPORTANCE: Zoonotic infection of humans with herpes B virus (BV) causes severe neurological diseases. Acyclovir (ACV) and ganciclovir (GCV), most frequently used as anti-herpes drugs, are recommended for prophylaxis and therapy in human BV infection. In this study, we examined the property of BV thymidine kinase (TK) against anti-herpes drugs using a recombinant herpes simplex virus type 1 (HSV-1) carrying BV TK gene. We found that HSV-1 carrying BV TK was similarly sensitive to GCV as HSV-1 carrying varicella zoster virus TK. In addition, we demonstrated that BV TK was not mutated in the GCV- and ACV-resistant HSV-1 carrying BV TK, suggesting that ACV- or GCV-resistant BV might be rare during treatment with these antiviral drugs. These data can provide a new insight into the properties of BV TK in terms of the development of drug resistance.

Original language	English (US)
Pages (from-to)	e0309123
Journal	Microbiology Spectrum
Volume	12
Issue number	1
DOIs	https://doi.org/10.1128/spectrum.03091-23
State	Published - Jan 11 2024

Keywords

B virus
DNA polymerase
acyclovir
acyclovir resistant
ganciclovir
ganciclovir resistant
herpes simplex virus 1
thymidine kinases

ASJC Scopus subject areas

Physiology
Ecology
General Immunology and Microbiology
Genetics
Microbiology (medical)
Cell Biology
Infectious Diseases

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10.1128/spectrum.03091-23

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title = "Analysis of antiviral drug properties of thymidine kinase of herpes B virus using recombinant herpes simplex virus 1",

abstract = "IMPORTANCE: Zoonotic infection of humans with herpes B virus (BV) causes severe neurological diseases. Acyclovir (ACV) and ganciclovir (GCV), most frequently used as anti-herpes drugs, are recommended for prophylaxis and therapy in human BV infection. In this study, we examined the property of BV thymidine kinase (TK) against anti-herpes drugs using a recombinant herpes simplex virus type 1 (HSV-1) carrying BV TK gene. We found that HSV-1 carrying BV TK was similarly sensitive to GCV as HSV-1 carrying varicella zoster virus TK. In addition, we demonstrated that BV TK was not mutated in the GCV- and ACV-resistant HSV-1 carrying BV TK, suggesting that ACV- or GCV-resistant BV might be rare during treatment with these antiviral drugs. These data can provide a new insight into the properties of BV TK in terms of the development of drug resistance.",

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doi = "10.1128/spectrum.03091-23",

language = "English (US)",

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AU - Nguyen, Phu Hoang Anh

AU - Fukushi, Shuetsu

AU - Yamada, Souichi

AU - Harada, Shizuko

AU - Yoshikawa, Tomoki

AU - Kinoshita, Hitomi

AU - Kawahara, Madoka

AU - Ogawa, Takuma

AU - Ebihara, Hideki

AU - Moi, Meng Ling

AU - Saijo, Masayuki

PY - 2024/1/11

Y1 - 2024/1/11

N2 - IMPORTANCE: Zoonotic infection of humans with herpes B virus (BV) causes severe neurological diseases. Acyclovir (ACV) and ganciclovir (GCV), most frequently used as anti-herpes drugs, are recommended for prophylaxis and therapy in human BV infection. In this study, we examined the property of BV thymidine kinase (TK) against anti-herpes drugs using a recombinant herpes simplex virus type 1 (HSV-1) carrying BV TK gene. We found that HSV-1 carrying BV TK was similarly sensitive to GCV as HSV-1 carrying varicella zoster virus TK. In addition, we demonstrated that BV TK was not mutated in the GCV- and ACV-resistant HSV-1 carrying BV TK, suggesting that ACV- or GCV-resistant BV might be rare during treatment with these antiviral drugs. These data can provide a new insight into the properties of BV TK in terms of the development of drug resistance.

AB - IMPORTANCE: Zoonotic infection of humans with herpes B virus (BV) causes severe neurological diseases. Acyclovir (ACV) and ganciclovir (GCV), most frequently used as anti-herpes drugs, are recommended for prophylaxis and therapy in human BV infection. In this study, we examined the property of BV thymidine kinase (TK) against anti-herpes drugs using a recombinant herpes simplex virus type 1 (HSV-1) carrying BV TK gene. We found that HSV-1 carrying BV TK was similarly sensitive to GCV as HSV-1 carrying varicella zoster virus TK. In addition, we demonstrated that BV TK was not mutated in the GCV- and ACV-resistant HSV-1 carrying BV TK, suggesting that ACV- or GCV-resistant BV might be rare during treatment with these antiviral drugs. These data can provide a new insight into the properties of BV TK in terms of the development of drug resistance.

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Analysis of antiviral drug properties of thymidine kinase of herpes B virus using recombinant herpes simplex virus 1

Abstract

Keywords

ASJC Scopus subject areas

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