Albumin In Situ Hybridization Can BePositive in Adenocarcinomas and OtherTumors From Diverse Sites

Aqsa Nasir, Heidi D. Lehrke, a. Taofic Mounajjed, Samar Said, Lizhi Zhang, Saba Yasir, Sejal S. Shah, Vishal S. Chandan, Thomas C. Smyrk, Roger K. Moreira

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Objectives Albumin messenger RNA (mRNA) expression is a marker ofhepatocellular differentiation. Most published data are fromreview of tissue microarrays, and albumin in situ hybridization(ISH) expression across several tumor types is incompletelycharacterized. Methods Sections from 221 tumors were evaluated for albumin mRNA.Immunohistochemistry was used to confirm diagnoses.Albumin ISH was performed according to manufacturer-provided instructions. Fifty-nine cases were evaluated with bothcommercial ISH assays. Results Albumin mRNA was detected in all hepatocellular carcinomas(HCCs) and 81% of intrahepatic cholangiocarcinomas. Lung(20%), gallbladder (39%), hepatoid pancreatic (n = 1 of 1)adenocarcinoma, breast invasive ductal carcinoma (18%), yolksac tumor (25%), and acinar cell carcinoma (29%) showedexpression. Both assays were concordant in 93% of cases. Conclusions Albumin ISH was expressed in all HCCs studied. It was alsopositive in intrahepatic cholangiocarcinoma and patchy positivein gallbladder adenocarcinoma and a subset of other neoplasms,which can be a potential pitfall.

Original languageEnglish (US)
Pages (from-to)190-199
Number of pages10
JournalAmerican journal of clinical pathology
Issue number2
StatePublished - 2019

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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