Adipose-Derived Biogenic Nanoparticles for Suppression of Inflammation

Ming Tian; Taylor Ticer; Qikun Wang; Sierra Walker; Anthony Pham; Annie Suh; Sara Busatto; Irina Davidovich; Rawan Al-Kharboosh; Laura Lewis-Tuffin; Baoan Ji; Alfredo Quinones-Hinojosa; Yeshayahu Talmon; Shane Shapiro; Felix Rückert; Joy Wolfram

doi:10.1002/smll.201904064

Adipose-Derived Biogenic Nanoparticles for Suppression of Inflammation

Ming Tian, Taylor Ticer, Qikun Wang, Sierra Walker, Anthony Pham, Annie Suh, Sara Busatto, Irina Davidovich, Rawan Al-Kharboosh, Laura Lewis-Tuffin, Baoan Ji, Alfredo Quinones-Hinojosa, Yeshayahu Talmon, Shane Shapiro, Felix Rückert, Joy Wolfram

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Extracellular vesicles secreted from adipose-derived mesenchymal stem cells (ADSCs) have therapeutic effects in inflammatory diseases. However, production of extracellular vesicles (EVs) from ADSCs is costly, inefficient, and time consuming. The anti-inflammatory properties of adipose tissue-derived EVs and other biogenic nanoparticles have not been explored. In this study, biogenic nanoparticles are obtained directly from lipoaspirate, an easily accessible and abundant source of biological material. Compared to ADSC-EVs, lipoaspirate nanoparticles (Lipo-NPs) take less time to process (hours compared to months) and cost less to produce (clinical-grade cell culture facilities are not required). The physicochemical characteristics and anti-inflammatory properties of Lipo-NPs are evaluated and compared to those of patient-matched ADSC-EVs. Moreover, guanabenz loading in Lipo-NPs is evaluated for enhanced anti-inflammatory effects. Apolipoprotein E and glycerolipids are enriched in Lipo-NPs compared to ADSC-EVs. Additionally, the uptake of Lipo-NPs in hepatocytes and macrophages is higher. Lipo-NPs and ADSC-EVs have comparable protective and anti-inflammatory effects. Specifically, Lipo-NPs reduce toll-like receptor 4-induced secretion of inflammatory cytokines in macrophages. Guanabenz-loaded Lipo-NPs further suppress inflammatory pathways, suggesting that this combination therapy can have promising applications for inflammatory diseases.

Original language	English (US)
Article number	1904064
Journal	Small
Volume	16
Issue number	10
DOIs	https://doi.org/10.1002/smll.201904064
State	Published - Mar 1 2020

Keywords

anti-inflammation
exosome
extracellular vesicles
inflammation
lipoaspirate
microvesicle

ASJC Scopus subject areas

General Chemistry
Engineering (miscellaneous)
Biotechnology
General Materials Science
Biomaterials

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title = "Adipose-Derived Biogenic Nanoparticles for Suppression of Inflammation",

abstract = "Extracellular vesicles secreted from adipose-derived mesenchymal stem cells (ADSCs) have therapeutic effects in inflammatory diseases. However, production of extracellular vesicles (EVs) from ADSCs is costly, inefficient, and time consuming. The anti-inflammatory properties of adipose tissue-derived EVs and other biogenic nanoparticles have not been explored. In this study, biogenic nanoparticles are obtained directly from lipoaspirate, an easily accessible and abundant source of biological material. Compared to ADSC-EVs, lipoaspirate nanoparticles (Lipo-NPs) take less time to process (hours compared to months) and cost less to produce (clinical-grade cell culture facilities are not required). The physicochemical characteristics and anti-inflammatory properties of Lipo-NPs are evaluated and compared to those of patient-matched ADSC-EVs. Moreover, guanabenz loading in Lipo-NPs is evaluated for enhanced anti-inflammatory effects. Apolipoprotein E and glycerolipids are enriched in Lipo-NPs compared to ADSC-EVs. Additionally, the uptake of Lipo-NPs in hepatocytes and macrophages is higher. Lipo-NPs and ADSC-EVs have comparable protective and anti-inflammatory effects. Specifically, Lipo-NPs reduce toll-like receptor 4-induced secretion of inflammatory cytokines in macrophages. Guanabenz-loaded Lipo-NPs further suppress inflammatory pathways, suggesting that this combination therapy can have promising applications for inflammatory diseases.",

keywords = "anti-inflammation, exosome, extracellular vesicles, inflammation, lipoaspirate, microvesicle",

author = "Ming Tian and Taylor Ticer and Qikun Wang and Sierra Walker and Anthony Pham and Annie Suh and Sara Busatto and Irina Davidovich and Rawan Al-Kharboosh and Laura Lewis-Tuffin and Baoan Ji and Alfredo Quinones-Hinojosa and Yeshayahu Talmon and Shane Shapiro and Felix R{\"u}ckert and Joy Wolfram",

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year = "2020",

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doi = "10.1002/smll.201904064",

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T1 - Adipose-Derived Biogenic Nanoparticles for Suppression of Inflammation

AU - Tian, Ming

AU - Ticer, Taylor

AU - Wang, Qikun

AU - Walker, Sierra

AU - Pham, Anthony

AU - Suh, Annie

AU - Busatto, Sara

AU - Davidovich, Irina

AU - Al-Kharboosh, Rawan

AU - Lewis-Tuffin, Laura

AU - Ji, Baoan

AU - Quinones-Hinojosa, Alfredo

AU - Talmon, Yeshayahu

AU - Shapiro, Shane

AU - Rückert, Felix

AU - Wolfram, Joy

PY - 2020/3/1

Y1 - 2020/3/1

N2 - Extracellular vesicles secreted from adipose-derived mesenchymal stem cells (ADSCs) have therapeutic effects in inflammatory diseases. However, production of extracellular vesicles (EVs) from ADSCs is costly, inefficient, and time consuming. The anti-inflammatory properties of adipose tissue-derived EVs and other biogenic nanoparticles have not been explored. In this study, biogenic nanoparticles are obtained directly from lipoaspirate, an easily accessible and abundant source of biological material. Compared to ADSC-EVs, lipoaspirate nanoparticles (Lipo-NPs) take less time to process (hours compared to months) and cost less to produce (clinical-grade cell culture facilities are not required). The physicochemical characteristics and anti-inflammatory properties of Lipo-NPs are evaluated and compared to those of patient-matched ADSC-EVs. Moreover, guanabenz loading in Lipo-NPs is evaluated for enhanced anti-inflammatory effects. Apolipoprotein E and glycerolipids are enriched in Lipo-NPs compared to ADSC-EVs. Additionally, the uptake of Lipo-NPs in hepatocytes and macrophages is higher. Lipo-NPs and ADSC-EVs have comparable protective and anti-inflammatory effects. Specifically, Lipo-NPs reduce toll-like receptor 4-induced secretion of inflammatory cytokines in macrophages. Guanabenz-loaded Lipo-NPs further suppress inflammatory pathways, suggesting that this combination therapy can have promising applications for inflammatory diseases.

AB - Extracellular vesicles secreted from adipose-derived mesenchymal stem cells (ADSCs) have therapeutic effects in inflammatory diseases. However, production of extracellular vesicles (EVs) from ADSCs is costly, inefficient, and time consuming. The anti-inflammatory properties of adipose tissue-derived EVs and other biogenic nanoparticles have not been explored. In this study, biogenic nanoparticles are obtained directly from lipoaspirate, an easily accessible and abundant source of biological material. Compared to ADSC-EVs, lipoaspirate nanoparticles (Lipo-NPs) take less time to process (hours compared to months) and cost less to produce (clinical-grade cell culture facilities are not required). The physicochemical characteristics and anti-inflammatory properties of Lipo-NPs are evaluated and compared to those of patient-matched ADSC-EVs. Moreover, guanabenz loading in Lipo-NPs is evaluated for enhanced anti-inflammatory effects. Apolipoprotein E and glycerolipids are enriched in Lipo-NPs compared to ADSC-EVs. Additionally, the uptake of Lipo-NPs in hepatocytes and macrophages is higher. Lipo-NPs and ADSC-EVs have comparable protective and anti-inflammatory effects. Specifically, Lipo-NPs reduce toll-like receptor 4-induced secretion of inflammatory cytokines in macrophages. Guanabenz-loaded Lipo-NPs further suppress inflammatory pathways, suggesting that this combination therapy can have promising applications for inflammatory diseases.

KW - anti-inflammation

KW - exosome

KW - extracellular vesicles

KW - inflammation

KW - lipoaspirate

KW - microvesicle

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DO - 10.1002/smll.201904064

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AN - SCOPUS:85079702954

SN - 1613-6810

VL - 16

JO - Small

JF - Small

IS - 10

M1 - 1904064

ER -

Adipose-Derived Biogenic Nanoparticles for Suppression of Inflammation

Abstract

Keywords

ASJC Scopus subject areas

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