Active antitumor immunity elicited by vaccine based on recombinant form of epidermal growth factor receptor

Bing Hu, Yuquan Wei, Ling Tian, Xia Zhao, You Lu, Yang Wu, Bing Yao, Jiyan Liu, Ting Niu, Yanjun Wen, Qiuming He, Jingmei Su, Meijuan Huang, Yanyan Lou, Yan Luo, Bing Kan

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Active immunotherapy targeting epidermal growth factor receptor (EGFR) should be another attractive approach to the treatment of EGFR-positive tumors. To test this concept, the authors evaluated the potential immune responses and antitumor activities elicited by dendritic cells pulsed with recombinant ectodomain of mouse EGFR (DC-edMER). Spleen cells isolated from DC-edMER-vaccinated mice showed a high quantity of EGFR-specific antibody-producing cells. EGFR-reactive antibody in sera isolated from vaccinated mice was identified and shown to be effective against tumors in vitro and in vivo by adoptive transfer. DC-edMER vaccine also elicited cytotoxic T-lymphocyte responses that could mediate antitumor effects in vitro and adoptive transfer in vivo. In addition, EGFR-specific cytokines responses were elicited by DC-edMER vaccine. Immunization with DC-edMER resulted in tumor regression and prolonged survival in mice challenged with Lewis lung carcinomas and mammary cancer models. Depletion of CD4+ T lymphocytes could completely abrogate the antitumor activity and EGFR-specific antibody responses, whereas the depletion of CD8+ T lymphocytes showed partial abrogation of the antitumor activity but antibody was still detected. Furthermore, tumor-induced angiogenesis was suppressed in DC-edMER-vaccinated mice or mice treated with antibody adoptive transfer. Taken together, these findings suggest the antitumor immunity could be induced by DC-edMER, which may involve both humoral and cellular immunity, and may provide insight into the treatment of EGFR-positive tumors through the induction of active immunity against EGFR.

Original languageEnglish (US)
Pages (from-to)236-244
Number of pages9
JournalJournal of Immunotherapy
Issue number3
StatePublished - 2005


  • Antitumor immunity
  • Dendritic cell
  • Epidermal growth factor receptor (EGFR)
  • Protein vaccine

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology
  • Cancer Research


Dive into the research topics of 'Active antitumor immunity elicited by vaccine based on recombinant form of epidermal growth factor receptor'. Together they form a unique fingerprint.

Cite this