TY - JOUR
T1 - Acquisition, Processing and Analysis of Electrocardiogram in Awake Zebrafish
AU - Le, Tai
AU - Lenning, Michael
AU - Clark, Isaac
AU - Bhimani, Ishaan
AU - Fortunato, Joseph
AU - Marsh, Paul
AU - Xu, Xiaolei
AU - Cao, Hung
N1 - Publisher Copyright:
© 2001-2012 IEEE.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Long-term monitoring of intrinsic electrocardiogram (ECG) in zebrafish plays a crucial role in heart disease studies and drug screening. In this paper, we developed a polymer-based apparatus with embedded flexible thin-film electrodes to acquire ECG signals of awake zebrafish. The apparatus was made of polydimethylsiloxane using the molding technique with molds formed by 3D printing. A graphical user interface was built in National Instruments LabView platform for real-time recording, processing, and analysis. The program provided important features, such as signal de-noising, characteristic wave detection, and anomaly detection. Further, it could operate on both real-time coming signals and previously-saved data, facilitating analysis and interpretation. We demonstrated the use of our system to investigate the effects of the anesthetic drug, namely Tricaine (MS-222), on cardiac electrophysiology of zebrafish, revealing promising findings. We speculate that our novel system may contribute to a host of studies in various disciplines using the zebrafish model.
AB - Long-term monitoring of intrinsic electrocardiogram (ECG) in zebrafish plays a crucial role in heart disease studies and drug screening. In this paper, we developed a polymer-based apparatus with embedded flexible thin-film electrodes to acquire ECG signals of awake zebrafish. The apparatus was made of polydimethylsiloxane using the molding technique with molds formed by 3D printing. A graphical user interface was built in National Instruments LabView platform for real-time recording, processing, and analysis. The program provided important features, such as signal de-noising, characteristic wave detection, and anomaly detection. Further, it could operate on both real-time coming signals and previously-saved data, facilitating analysis and interpretation. We demonstrated the use of our system to investigate the effects of the anesthetic drug, namely Tricaine (MS-222), on cardiac electrophysiology of zebrafish, revealing promising findings. We speculate that our novel system may contribute to a host of studies in various disciplines using the zebrafish model.
KW - Zebrafish
KW - automated processing
KW - electrocardiogram (ECG)
KW - heart disease
KW - labview
UR - http://www.scopus.com/inward/record.url?scp=85065389369&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85065389369&partnerID=8YFLogxK
U2 - 10.1109/JSEN.2019.2897789
DO - 10.1109/JSEN.2019.2897789
M3 - Article
AN - SCOPUS:85065389369
SN - 1530-437X
VL - 19
SP - 4283
EP - 4289
JO - IEEE Sensors Journal
JF - IEEE Sensors Journal
IS - 11
M1 - 8636511
ER -