TY - JOUR
T1 - AcMNPV ORF38 protein has the activity of ADP-ribose pyrophosphatase and is important for virus replication
AU - Ge, Jing
AU - Wei, Zong
AU - Huang, Yishu
AU - Yin, Juan
AU - Zhou, Ziqian
AU - Zhong, Jiang
N1 - Funding Information:
This work is supported by the National Natural Science Foundation of China projects number 30370050 and 30070031.
PY - 2007/4/25
Y1 - 2007/4/25
N2 - The ORF38 of Autographa californica multiple nucleopolyhedrovirus (AcMNPV), or AcORF38, contains a conserved motif of Nudix (nucleotide diphosphate X) superfamily. It has the highest homology with ADP-ribose pyrophosphatase (ADPRase), a subfamily of Nudix pyrophosphatase. In the current study, recombinant AcORF38 protein was prepared and shown to have ADPRase activity, with a Km of 204 μM, and Kcat of 6.96 s- 1 at pH 8.0 and 5 mM MgCl2. The transcription of AcORF38 was detected 2 h postinfection, and lasted until the late stage. An orf38 gene-deleted mutant virus, vAcGFP-Δ38, was constructed. Although it produced progeny virus, the yield of extracellular virus was less than 1% of the wild-type virus. The activity of viral very late gene promoter was also greatly reduced in vAcGFP-Δ38-infected cells as indicated by the expression of green fluorescence protein gene driven by polyhedrin promoter. The mutant phenotype was rescued by co-transfection with an AcORF38-expressing plasmid. These results suggest that AcORF38 plays an important role in virus replication, although the detail is to be elucidated.
AB - The ORF38 of Autographa californica multiple nucleopolyhedrovirus (AcMNPV), or AcORF38, contains a conserved motif of Nudix (nucleotide diphosphate X) superfamily. It has the highest homology with ADP-ribose pyrophosphatase (ADPRase), a subfamily of Nudix pyrophosphatase. In the current study, recombinant AcORF38 protein was prepared and shown to have ADPRase activity, with a Km of 204 μM, and Kcat of 6.96 s- 1 at pH 8.0 and 5 mM MgCl2. The transcription of AcORF38 was detected 2 h postinfection, and lasted until the late stage. An orf38 gene-deleted mutant virus, vAcGFP-Δ38, was constructed. Although it produced progeny virus, the yield of extracellular virus was less than 1% of the wild-type virus. The activity of viral very late gene promoter was also greatly reduced in vAcGFP-Δ38-infected cells as indicated by the expression of green fluorescence protein gene driven by polyhedrin promoter. The mutant phenotype was rescued by co-transfection with an AcORF38-expressing plasmid. These results suggest that AcORF38 plays an important role in virus replication, although the detail is to be elucidated.
KW - ADP-ribose pyrophosphatase
KW - AcMNPV
KW - Baculovirus
KW - Nudix pyrophosphatase
KW - ORF38
KW - Virus replication
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U2 - 10.1016/j.virol.2006.11.017
DO - 10.1016/j.virol.2006.11.017
M3 - Article
C2 - 17174373
AN - SCOPUS:34047266624
SN - 0042-6822
VL - 361
SP - 204
EP - 211
JO - Virology
JF - Virology
IS - 1
ER -