Acetylcholinesterase immunolesioning: Regional vulnerability of preganglionic sympathetic neurons in rat spinal cord

Hui Tang, Pamela Hammond, Stephen Brimijoin

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Rats given antibodies against acetylcholinesterase (ACHE) develop sympathetic dysfunction stemming from losses of preganglionic neurons in spinal cord. Central effects of AChE antibodies are surprising since IgG does not readily cross the blood-brain barrier, and lesions of peripheral terminals should not cause cell death. This study was designed to explore the distribution of central neural damage and to investigate features that might account for vulnerability. Rat spinal cord and brainstem were stained for choline acetyltransferase (CHAT) and nitric oxide synthase (NOS) immunoreactivity. Four months after administration of AChE antibodies, ChAT- positive neurons in the intermediolateral nucleus (IML) were 61-66% fewer throughout the thoracolumbar cord (T1, T2, T8, T12, L1). NOS-positive neurons in these loci were affected to the same extent by antibody-treatment, although they were only two-thirds as numerous. By contrast, neurons in the central autonomic nucleus of the thoracolumbar cord were scarcely affected. These results point to immunochemical differences in the central autonomic outflow, which may partially explain the puzzling selectivity of neural damage in AChE immunolesioning. Different results were obtained after guanethidine sympathectomy, which ablated nearly all neurons in the superior cervical ganglion without any effect on preganglionic neurons in the IML. Therefore, if the central effects of antibodies are indirectly mediated by loss of trophic support from the periphery, this sup, port cannot arise from adrenergic neurons but must come from other ganglionic cells.

Original languageEnglish (US)
Pages (from-to)167-176
Number of pages10
JournalExperimental Neurology
Issue number2
StatePublished - Aug 1998


  • Central autonomic neurons
  • Guanethidine
  • Intermediolateral nucleus
  • Neuronal death
  • Parasympathetic system
  • Sympathectomy

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience


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