Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles

Sean Wei Xiang Ong; Pei Yun Hon; Sharon Syn Hui Wee; Jonathan Wei Zhong Chia; Shehara Mendis; Ezlyn Izharuddin; Ray Junhao Lin; Po Ying Chia; Rees Chin Swee Sim; Mark I.Cheng Chen; Angela Chow; Joanne Yoong; David Chien Lye; Christine B. Teng; Paul Anantharajah Tambyah; Ritu Banerjee; Robin Patel; Partha Pratim De; Shawn Vasoo

doi:10.1093/cid/ciab848

Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles

Sean Wei Xiang Ong, Pei Yun Hon, Sharon Syn Hui Wee, Jonathan Wei Zhong Chia, Shehara Mendis, Ezlyn Izharuddin, Ray Junhao Lin, Po Ying Chia, Rees Chin Swee Sim, Mark I.Cheng Chen, Angela Chow, Joanne Yoong, David Chien Lye, Christine B. Teng, Paul Anantharajah Tambyah, Ritu Banerjee, Robin Patel, Partha Pratim De, Shawn Vasoo

Research output: Contribution to journal › Article › peer-review

Abstract

We studied the performance of an algorithm combining multiplex polymerase chain reaction with phenotypic detection of extended-spectrum β-lactamases and carbapenemases directly from positive blood culture bottles in patients with gram-negative bacteremia and found good concordance with routine cultures. Such an algorithm may be a tool to improve time to optimal therapy in patients with gram-negative bacteremia.

Original language	English (US)
Pages (from-to)	1850-1854
Number of pages	5
Journal	Clinical Infectious Diseases
Volume	74
Issue number	10
DOIs	https://doi.org/10.1093/cid/ciab848
State	Published - May 15 2022

Keywords

ESBL
antimicrobial resistance
carbapenemase
diagnostics
gram-negative bacteria
rapid test kits

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

Access to Document

10.1093/cid/ciab848

Fingerprint

Dive into the research topics of 'Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles'. Together they form a unique fingerprint.

Cite this

Ong, S. W. X., Hon, P. Y., Wee, S. S. H., Chia, J. W. Z., Mendis, S., Izharuddin, E., Lin, R. J., Chia, P. Y., Sim, R. C. S., Chen, M. I. C., Chow, A., Yoong, J., Lye, D. C., Teng, C. B., Tambyah, P. A., Banerjee, R., Patel, R., De, P. P., & Vasoo, S. (2022). Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles. Clinical Infectious Diseases, 74(10), 1850-1854. https://doi.org/10.1093/cid/ciab848

Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles. / Ong, Sean Wei Xiang; Hon, Pei Yun; Wee, Sharon Syn Hui et al.
In: Clinical Infectious Diseases, Vol. 74, No. 10, 15.05.2022, p. 1850-1854.

Research output: Contribution to journal › Article › peer-review

Ong, SWX, Hon, PY, Wee, SSH, Chia, JWZ, Mendis, S, Izharuddin, E, Lin, RJ, Chia, PY, Sim, RCS, Chen, MIC, Chow, A, Yoong, J, Lye, DC, Teng, CB, Tambyah, PA, Banerjee, R, Patel, R, De, PP & Vasoo, S 2022, 'Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles', Clinical Infectious Diseases, vol. 74, no. 10, pp. 1850-1854. https://doi.org/10.1093/cid/ciab848

Ong SWX, Hon PY, Wee SSH, Chia JWZ, Mendis S, Izharuddin E et al. Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles. Clinical Infectious Diseases. 2022 May 15;74(10):1850-1854. doi: 10.1093/cid/ciab848

Ong, Sean Wei Xiang ; Hon, Pei Yun ; Wee, Sharon Syn Hui et al. / Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles. In: Clinical Infectious Diseases. 2022 ; Vol. 74, No. 10. pp. 1850-1854.

@article{f4d5e96ee1854bb18e0879237db89a22,

title = "Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles",

abstract = "We studied the performance of an algorithm combining multiplex polymerase chain reaction with phenotypic detection of extended-spectrum β-lactamases and carbapenemases directly from positive blood culture bottles in patients with gram-negative bacteremia and found good concordance with routine cultures. Such an algorithm may be a tool to improve time to optimal therapy in patients with gram-negative bacteremia.",

keywords = "ESBL, antimicrobial resistance, carbapenemase, diagnostics, gram-negative bacteria, rapid test kits",

author = "Ong, {Sean Wei Xiang} and Hon, {Pei Yun} and Wee, {Sharon Syn Hui} and Chia, {Jonathan Wei Zhong} and Shehara Mendis and Ezlyn Izharuddin and Lin, {Ray Junhao} and Chia, {Po Ying} and Sim, {Rees Chin Swee} and Chen, {Mark I.Cheng} and Angela Chow and Joanne Yoong and Lye, {David Chien} and Teng, {Christine B.} and Tambyah, {Paul Anantharajah} and Ritu Banerjee and Robin Patel and De, {Partha Pratim} and Shawn Vasoo",

year = "2022",

month = may,

day = "15",

doi = "10.1093/cid/ciab848",

language = "English (US)",

volume = "74",

pages = "1850--1854",

journal = "Clinical Infectious Diseases",

issn = "1058-4838",

publisher = "Oxford University Press",

number = "10",

}

TY - JOUR

T1 - Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles

AU - Ong, Sean Wei Xiang

AU - Hon, Pei Yun

AU - Wee, Sharon Syn Hui

AU - Chia, Jonathan Wei Zhong

AU - Mendis, Shehara

AU - Izharuddin, Ezlyn

AU - Lin, Ray Junhao

AU - Chia, Po Ying

AU - Sim, Rees Chin Swee

AU - Chen, Mark I.Cheng

AU - Chow, Angela

AU - Yoong, Joanne

AU - Lye, David Chien

AU - Teng, Christine B.

AU - Tambyah, Paul Anantharajah

AU - Banerjee, Ritu

AU - Patel, Robin

AU - De, Partha Pratim

AU - Vasoo, Shawn

PY - 2022/5/15

Y1 - 2022/5/15

N2 - We studied the performance of an algorithm combining multiplex polymerase chain reaction with phenotypic detection of extended-spectrum β-lactamases and carbapenemases directly from positive blood culture bottles in patients with gram-negative bacteremia and found good concordance with routine cultures. Such an algorithm may be a tool to improve time to optimal therapy in patients with gram-negative bacteremia.

AB - We studied the performance of an algorithm combining multiplex polymerase chain reaction with phenotypic detection of extended-spectrum β-lactamases and carbapenemases directly from positive blood culture bottles in patients with gram-negative bacteremia and found good concordance with routine cultures. Such an algorithm may be a tool to improve time to optimal therapy in patients with gram-negative bacteremia.

KW - ESBL

KW - antimicrobial resistance

KW - carbapenemase

KW - diagnostics

KW - gram-negative bacteria

KW - rapid test kits

UR - http://www.scopus.com/inward/record.url?scp=85131222638&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85131222638&partnerID=8YFLogxK

U2 - 10.1093/cid/ciab848

DO - 10.1093/cid/ciab848

M3 - Article

C2 - 34554228

AN - SCOPUS:85131222638

SN - 1058-4838

VL - 74

SP - 1850

EP - 1854

JO - Clinical Infectious Diseases

JF - Clinical Infectious Diseases

IS - 10

ER -

Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this