Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles

Sean Wei Xiang Ong; Pei Yun Hon; Sharon Syn Hui Wee; Jonathan Wei Zhong Chia; Shehara Mendis; Ezlyn Izharuddin; Ray Junhao Lin; Po Ying Chia; Rees Chin Swee Sim; Mark I.Cheng Chen; Angela Chow; Joanne Yoong; David Chien Lye; Christine B. Teng; Paul Anantharajah Tambyah; Ritu Banerjee; Robin Patel; Partha Pratim De; Shawn Vasoo

doi:10.1093/cid/ciab848

Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles

Sean Wei Xiang Ong, Pei Yun Hon, Sharon Syn Hui Wee, Jonathan Wei Zhong Chia, Shehara Mendis, Ezlyn Izharuddin, Ray Junhao Lin, Po Ying Chia, Rees Chin Swee Sim, Mark I.Cheng Chen, Angela Chow, Joanne Yoong, David Chien Lye, Christine B. Teng, Paul Anantharajah Tambyah, Ritu Banerjee, Robin Patel, Partha Pratim De, Shawn Vasoo

Research output: Contribution to journal › Article › peer-review

Abstract

We studied the performance of an algorithm combining multiplex polymerase chain reaction with phenotypic detection of extended-spectrum β-lactamases and carbapenemases directly from positive blood culture bottles in patients with gram-negative bacteremia and found good concordance with routine cultures. Such an algorithm may be a tool to improve time to optimal therapy in patients with gram-negative bacteremia.

Original language	English (US)
Pages (from-to)	1850-1854
Number of pages	5
Journal	Clinical Infectious Diseases
Volume	74
Issue number	10
DOIs	https://doi.org/10.1093/cid/ciab848
State	Published - May 15 2022

Keywords

ESBL
antimicrobial resistance
carbapenemase
diagnostics
gram-negative bacteria
rapid test kits

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

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Ong, S. W. X., Hon, P. Y., Wee, S. S. H., Chia, J. W. Z., Mendis, S., Izharuddin, E., Lin, R. J., Chia, P. Y., Sim, R. C. S., Chen, M. I. C., Chow, A., Yoong, J., Lye, D. C., Teng, C. B., Tambyah, P. A., Banerjee, R., Patel, R., De, P. P., & Vasoo, S. (2022). Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles. Clinical Infectious Diseases, 74(10), 1850-1854. https://doi.org/10.1093/cid/ciab848

Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles. / Ong, Sean Wei Xiang; Hon, Pei Yun; Wee, Sharon Syn Hui et al.
In: Clinical Infectious Diseases, Vol. 74, No. 10, 15.05.2022, p. 1850-1854.

Research output: Contribution to journal › Article › peer-review

Ong, SWX, Hon, PY, Wee, SSH, Chia, JWZ, Mendis, S, Izharuddin, E, Lin, RJ, Chia, PY, Sim, RCS, Chen, MIC, Chow, A, Yoong, J, Lye, DC, Teng, CB, Tambyah, PA, Banerjee, R, Patel, R, De, PP & Vasoo, S 2022, 'Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles', Clinical Infectious Diseases, vol. 74, no. 10, pp. 1850-1854. https://doi.org/10.1093/cid/ciab848

Ong SWX, Hon PY, Wee SSH, Chia JWZ, Mendis S, Izharuddin E et al. Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles. Clinical Infectious Diseases. 2022 May 15;74(10):1850-1854. doi: 10.1093/cid/ciab848

Ong, Sean Wei Xiang ; Hon, Pei Yun ; Wee, Sharon Syn Hui et al. / Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles. In: Clinical Infectious Diseases. 2022 ; Vol. 74, No. 10. pp. 1850-1854.

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abstract = "We studied the performance of an algorithm combining multiplex polymerase chain reaction with phenotypic detection of extended-spectrum β-lactamases and carbapenemases directly from positive blood culture bottles in patients with gram-negative bacteremia and found good concordance with routine cultures. Such an algorithm may be a tool to improve time to optimal therapy in patients with gram-negative bacteremia.",

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Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles

Abstract

Keywords

ASJC Scopus subject areas

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