A Versatile Protocol for Studying Calvarial Bone Defect Healing in a Mouse Model

Rebekah M. Samsonraj, Amel Dudakovic, Pengfei Zan, Oksana Pichurin, Simon M. Cool, Andre J. Van Wijnen

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Animal models are vital tools for the preclinical development and testing of therapies aimed at providing solutions for several musculoskeletal disorders. For bone tissue engineering strategies addressing nonunion conditions, rodent models are particularly useful for studying bone healing in a controlled environment. The mouse calvarial defect model permits evaluation of drug, growth factor, or cell transplantation efficacy, together with offering the benefit of utilizing genetic models to study intramembranous bone formation within defect sites. In this study, we describe a detailed methodology for creating calvarial defects in mouse and present our results on bone morphogenetic protein-2-loaded fibrin scaffolds, thus advocating the utility of this functional orthotopic mouse model for the evaluation of therapeutic interventions (such as growth factors or cells) intended for successful bone regeneration therapies.

Original languageEnglish (US)
Pages (from-to)686-693
Number of pages8
JournalTissue Engineering - Part C: Methods
Issue number11
StatePublished - Nov 2017


  • animal model
  • bone regeneration
  • calvaria
  • calvarial defect
  • mouse/mice

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering


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