A two-center international evaluation of the Immulite 2000 automated serum gastrin assay

Mary L. Eastvold; Hans W. Wallinder; Carol M. Preissner; Ravinder J. Singh; Christian Löwbeer; Stefan K.G. Grebe

doi:10.1016/j.clinbiochem.2006.01.004

A two-center international evaluation of the Immulite 2000 automated serum gastrin assay

Mary L. Eastvold, Hans W. Wallinder, Carol M. Preissner, Ravinder J. Singh, Christian Löwbeer, Stefan K.G. Grebe

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Objectives: To assess the performance of the Immulite 2000 serum gastrin assay. Design and methods: Two-center validation and comparison with two manual gastrin assays. Results: Serum, serum separator, EDTA and heparin tube results differed <20%. Samples showed <20% degradation for <6 h at room temperature, 3-day-refrigerated, and underwent 3 freeze-thaw cycles. Imprecision was <8% between 20 and 824 ng/L (10-393 pmol/L). Spike recovery was 88.5-106%, and recovery on dilution was 62-135%. There were no interferences from gastrin 1-14, pentagastrin, and cholecystokinin (CCK) 1-33 and CCK 26-33. The fasting reference range was <100 ng/L (<48 pmol/L). Regression slopes to the manual assays were 0.9 and 0.97, with comparable clinical performance. Conclusions: The Immulite 2000 assay is precise, accurate, and fast and compares well with established gastrin assays.

Original language	English (US)
Pages (from-to)	387-390
Number of pages	4
Journal	Clinical Biochemistry
Volume	39
Issue number	4
DOIs	https://doi.org/10.1016/j.clinbiochem.2006.01.004
State	Published - Apr 1 2006

Keywords

Automated chemiluminometric immunoassay
Gastrin
Gastrinoma
Pernicious anemia
Zollinger-Ellison syndrome

ASJC Scopus subject areas

Clinical Biochemistry

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10.1016/j.clinbiochem.2006.01.004

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title = "A two-center international evaluation of the Immulite 2000 automated serum gastrin assay",

abstract = "Objectives: To assess the performance of the Immulite 2000 serum gastrin assay. Design and methods: Two-center validation and comparison with two manual gastrin assays. Results: Serum, serum separator, EDTA and heparin tube results differed <20%. Samples showed <20% degradation for <6 h at room temperature, 3-day-refrigerated, and underwent 3 freeze-thaw cycles. Imprecision was <8% between 20 and 824 ng/L (10-393 pmol/L). Spike recovery was 88.5-106%, and recovery on dilution was 62-135%. There were no interferences from gastrin 1-14, pentagastrin, and cholecystokinin (CCK) 1-33 and CCK 26-33. The fasting reference range was <100 ng/L (<48 pmol/L). Regression slopes to the manual assays were 0.9 and 0.97, with comparable clinical performance. Conclusions: The Immulite 2000 assay is precise, accurate, and fast and compares well with established gastrin assays.",

keywords = "Automated chemiluminometric immunoassay, Gastrin, Gastrinoma, Pernicious anemia, Zollinger-Ellison syndrome",

author = "Eastvold, {Mary L.} and Wallinder, {Hans W.} and Preissner, {Carol M.} and Singh, {Ravinder J.} and Christian L{\"o}wbeer and Grebe, {Stefan K.G.}",

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AU - Eastvold, Mary L.

AU - Wallinder, Hans W.

AU - Preissner, Carol M.

AU - Singh, Ravinder J.

AU - Löwbeer, Christian

AU - Grebe, Stefan K.G.

PY - 2006/4/1

Y1 - 2006/4/1

N2 - Objectives: To assess the performance of the Immulite 2000 serum gastrin assay. Design and methods: Two-center validation and comparison with two manual gastrin assays. Results: Serum, serum separator, EDTA and heparin tube results differed <20%. Samples showed <20% degradation for <6 h at room temperature, 3-day-refrigerated, and underwent 3 freeze-thaw cycles. Imprecision was <8% between 20 and 824 ng/L (10-393 pmol/L). Spike recovery was 88.5-106%, and recovery on dilution was 62-135%. There were no interferences from gastrin 1-14, pentagastrin, and cholecystokinin (CCK) 1-33 and CCK 26-33. The fasting reference range was <100 ng/L (<48 pmol/L). Regression slopes to the manual assays were 0.9 and 0.97, with comparable clinical performance. Conclusions: The Immulite 2000 assay is precise, accurate, and fast and compares well with established gastrin assays.

AB - Objectives: To assess the performance of the Immulite 2000 serum gastrin assay. Design and methods: Two-center validation and comparison with two manual gastrin assays. Results: Serum, serum separator, EDTA and heparin tube results differed <20%. Samples showed <20% degradation for <6 h at room temperature, 3-day-refrigerated, and underwent 3 freeze-thaw cycles. Imprecision was <8% between 20 and 824 ng/L (10-393 pmol/L). Spike recovery was 88.5-106%, and recovery on dilution was 62-135%. There were no interferences from gastrin 1-14, pentagastrin, and cholecystokinin (CCK) 1-33 and CCK 26-33. The fasting reference range was <100 ng/L (<48 pmol/L). Regression slopes to the manual assays were 0.9 and 0.97, with comparable clinical performance. Conclusions: The Immulite 2000 assay is precise, accurate, and fast and compares well with established gastrin assays.

KW - Automated chemiluminometric immunoassay

KW - Gastrin

KW - Gastrinoma

KW - Pernicious anemia

KW - Zollinger-Ellison syndrome

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A two-center international evaluation of the Immulite 2000 automated serum gastrin assay

Abstract

Keywords

ASJC Scopus subject areas

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