Abstract
Exercise capacity, measured by treadmill in humans and other mammals, is an important diagnostic and prognostic index for patients with cardiomyopathy and heart failure. The adult zebrafish is increasingly used as a vertebrate model to study human cardiomyopathy due to its conserved cardiovascular physiology, convenience for genetic manipulation, and amenability to high-throughput genetic and compound screening. Owing to the small size of its body and heart, new phenotyping assays are needed to unveil phenotypic traits of cardiomyopathy in adult zebrafish. Here, we describe a swimming-based functional assay that measures exercise capacity in an adult zebrafish doxorubicin-induced cardiomyopathy model. This protocol can be applied to any adult zebrafish model of acquired or inherited cardiomyopathy and potentially to other cardiovascular diseases.
| Original language | English (US) |
|---|---|
| Article number | e4114 |
| Journal | Bio-protocol |
| Volume | 11 |
| Issue number | 15 |
| DOIs | |
| State | Published - Aug 5 2021 |
Keywords
- Adult Zebrafish
- Cardiomyopathy Model
- Critical Swimming Speed
- Doxorubicin-induced Cardiomyopathy
- Exercise Capacity
- Exercise Intolerance
- Heart Failure
- Swimming Tunnel
ASJC Scopus subject areas
- General Immunology and Microbiology
- General Biochemistry, Genetics and Molecular Biology
- General Neuroscience
- Plant Science