A role for LEDGF/p75 in targeting HIV DNA integration

Angela Ciuffi, Manuel Llano, Eric Poeschla, Christian Hoffmann, Jeremy Leipzig, Paul Shinn, Joseph R. Ecker, Frederic Bushman

Research output: Contribution to journalArticlepeer-review

449 Scopus citations


HIV DNA integration is favored in active genes, but the underlying mechanism is unclear. Cellular lens epithelium-derived growth factor (LEDGF/p75) binds both chromosomal DNA and HIV integrase, and might therefore direct integration by a tethering interaction. We analyzed HIV integration in cells depleted for LEDGF/p75, and found that integration was (i) less frequent in transcription units, (ii) less frequent in genes regulated by LEDGF/p75 and (iii) more frequent in GC-rich DNA. LEDGF is thus the first example of a cellular protein controlling the location of HIV integration in human cells.

Original languageEnglish (US)
Pages (from-to)1287-1289
Number of pages3
JournalNature Medicine
Issue number12
StatePublished - Dec 2005

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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