Abstract
Selection of target substrates by protein kinases is strongly influenced by the amino acid sequence surrounding the phosphoacceptor site. Identification of the preferred peptide phosphorylation motif for a given kinase permits the production of efficient peptide substrates and greatly simplifies the mapping of phosphorylation sites in protein substrates. Here we describe a combinatorial peptide library method that allows rapid generation of phosphorylation motifs for serine/threonine kinases.
Original language | English (US) |
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Pages (from-to) | 27-29 |
Number of pages | 3 |
Journal | Nature Methods |
Volume | 1 |
Issue number | 1 |
DOIs | |
State | Published - Oct 2004 |
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Cell Biology