A proteogenomic approach to map the proteome of an unsequenced pathogen - Leishmania donovani

Harsh Pawar, Nandini A. Sahasrabuddhe, Santosh Renuse, Shivakumar Keerthikumar, Jyoti Sharma, Ghantasala S.Sameer Kumar, Abhilash Venugopal, Nirujogi Raja Sekhar, Dhanashree S. Kelkar, Harshal Nemade, Sweta N. Khobragade, Babylakshmi Muthusamy, Kumaran Kandasamy, H. C. Harsha, Raghothama Chaerkady, Milind S. Patole, Akhilesh Pandey

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


Visceral leishmaniasis or kala azar is the most severe form of leishmaniasis and is caused by the protozoan parasite Leishmania donovani. There is no published report on L. donovani genome sequence available till date, although the genome sequences of three related Leishmania species are already available. Thus, we took a proteogenomic approach to identify proteins from two different life stages of L. donovani. From our analysis of the promastigote (insect) and amastigote (human) stages of L. donovani, we identified a total of 22322 unique peptides from a homology-based search against proteins from three Leishmania species. These peptides were assigned to 3711 proteins in L. infantum, 3287 proteins in L. major, and 2433 proteins in L. braziliensis. Of the 3711 L. donovani proteins that were identified, the expression of 1387 proteins was detectable in both life stages of the parasite, while 901 and 1423 proteins were identified only in promastigotes and amastigotes life stages, respectively. In addition, we also identified 13 N-terminally and one C-terminally extended proteins based on the proteomic data search against the six-frame translated genome of the three related Leishmania species. Here, we report results from proteomic profiling of L. donovani, an organism with an unsequenced genome.

Original languageEnglish (US)
Pages (from-to)832-844
Number of pages13
Issue number6
StatePublished - Mar 2012


  • Comparative proteogenomics
  • Digenic parasite
  • Kinetoplastid
  • Microbiology
  • Orthologs
  • Paralogs

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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