TY - JOUR
T1 - A proteogenomic approach to map the proteome of an unsequenced pathogen - Leishmania donovani
AU - Pawar, Harsh
AU - Sahasrabuddhe, Nandini A.
AU - Renuse, Santosh
AU - Keerthikumar, Shivakumar
AU - Sharma, Jyoti
AU - Kumar, Ghantasala S.Sameer
AU - Venugopal, Abhilash
AU - Sekhar, Nirujogi Raja
AU - Kelkar, Dhanashree S.
AU - Nemade, Harshal
AU - Khobragade, Sweta N.
AU - Muthusamy, Babylakshmi
AU - Kandasamy, Kumaran
AU - Harsha, H. C.
AU - Chaerkady, Raghothama
AU - Patole, Milind S.
AU - Pandey, Akhilesh
PY - 2012/3
Y1 - 2012/3
N2 - Visceral leishmaniasis or kala azar is the most severe form of leishmaniasis and is caused by the protozoan parasite Leishmania donovani. There is no published report on L. donovani genome sequence available till date, although the genome sequences of three related Leishmania species are already available. Thus, we took a proteogenomic approach to identify proteins from two different life stages of L. donovani. From our analysis of the promastigote (insect) and amastigote (human) stages of L. donovani, we identified a total of 22322 unique peptides from a homology-based search against proteins from three Leishmania species. These peptides were assigned to 3711 proteins in L. infantum, 3287 proteins in L. major, and 2433 proteins in L. braziliensis. Of the 3711 L. donovani proteins that were identified, the expression of 1387 proteins was detectable in both life stages of the parasite, while 901 and 1423 proteins were identified only in promastigotes and amastigotes life stages, respectively. In addition, we also identified 13 N-terminally and one C-terminally extended proteins based on the proteomic data search against the six-frame translated genome of the three related Leishmania species. Here, we report results from proteomic profiling of L. donovani, an organism with an unsequenced genome.
AB - Visceral leishmaniasis or kala azar is the most severe form of leishmaniasis and is caused by the protozoan parasite Leishmania donovani. There is no published report on L. donovani genome sequence available till date, although the genome sequences of three related Leishmania species are already available. Thus, we took a proteogenomic approach to identify proteins from two different life stages of L. donovani. From our analysis of the promastigote (insect) and amastigote (human) stages of L. donovani, we identified a total of 22322 unique peptides from a homology-based search against proteins from three Leishmania species. These peptides were assigned to 3711 proteins in L. infantum, 3287 proteins in L. major, and 2433 proteins in L. braziliensis. Of the 3711 L. donovani proteins that were identified, the expression of 1387 proteins was detectable in both life stages of the parasite, while 901 and 1423 proteins were identified only in promastigotes and amastigotes life stages, respectively. In addition, we also identified 13 N-terminally and one C-terminally extended proteins based on the proteomic data search against the six-frame translated genome of the three related Leishmania species. Here, we report results from proteomic profiling of L. donovani, an organism with an unsequenced genome.
KW - Comparative proteogenomics
KW - Digenic parasite
KW - Kinetoplastid
KW - Microbiology
KW - Orthologs
KW - Paralogs
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U2 - 10.1002/pmic.201100505
DO - 10.1002/pmic.201100505
M3 - Article
C2 - 22539434
AN - SCOPUS:84860316503
SN - 1615-9853
VL - 12
SP - 832
EP - 844
JO - Proteomics
JF - Proteomics
IS - 6
ER -