A novel aptamer developed for breast cancer cell internalization

Kejing Zhang, Kwame Sefah, Lili Tang, Zilong Zhao, Guizhi Zhu, Mao Ye, Weijia Sun, Steve Goodison, Weihong Tan

Research output: Contribution to journalArticlepeer-review

80 Scopus citations


Breast cancer affects one in eight women in the United States, with a mortality rate that is second only to lung cancer. Although chemotherapy is widely used in breast cancer treatment, its side effects remain a challenge. One way to address this problem is through drug delivery by the internalization of cell-type-specific probes. Although nucleic acid aptamers are excellent probes for molecular recognition, only a few studies have demonstrated that aptamers can be internalized into living cells. Therefore, herein we report the development of a cancer-cell-specific DNA aptamer probe, KMF2-1a. By using the cell-SELEX method, this aptamer was selected against breast cancer cell line MCF-10AT1. Our results show that KMF2-1a is internalized efficiently and specifically to the endosome of target breast cancer cells. These results indicate that KMF2-1a is a promising agent for cell-type-specific intracellular delivery with both diagnostic and therapeutic implications.

Original languageEnglish (US)
Pages (from-to)79-84
Number of pages6
Issue number1
StatePublished - Jan 2 2012


  • Aptamers
  • Breast cancer
  • Drug delivery
  • Internalization
  • MCF-10AT1

ASJC Scopus subject areas

  • Drug Discovery
  • General Pharmacology, Toxicology and Pharmaceutics
  • Molecular Medicine
  • Biochemistry
  • Pharmacology
  • Organic Chemistry


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