A non-rational approach to optimized targeting of CRISPR-Cas9 complexes

Brandon Wilbanks; Keenan Pearson; L. James Maher

doi:10.1016/j.chembiol.2023.07.012

A non-rational approach to optimized targeting of CRISPR-Cas9 complexes

Brandon Wilbanks, Keenan Pearson, L. James Maher

Biochemistry and Molecular Biology

Research output: Contribution to journal › Comment/debate › peer-review

Abstract

In this issue of Cell Chemical Biology, Bush et al.¹ report an in vitro selection method for optimizing CRISPR-Cas9 single-guide RNAs. This approach may be useful in targeting previously intractable genomic sequences. The results also provide insights into which positions in single-guide RNAs are most amenable to modification.

Original language	English (US)
Pages (from-to)	855-857
Number of pages	3
Journal	Cell Chemical Biology
Volume	30
Issue number	8
DOIs	https://doi.org/10.1016/j.chembiol.2023.07.012
State	Published - Aug 17 2023

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmacology
Drug Discovery
Clinical Biochemistry

Access to Document

10.1016/j.chembiol.2023.07.012

Cite this

@article{c86963181407498591c2e3f17d646972,

title = "A non-rational approach to optimized targeting of CRISPR-Cas9 complexes",

abstract = "In this issue of Cell Chemical Biology, Bush et al.1 report an in vitro selection method for optimizing CRISPR-Cas9 single-guide RNAs. This approach may be useful in targeting previously intractable genomic sequences. The results also provide insights into which positions in single-guide RNAs are most amenable to modification.",

author = "Brandon Wilbanks and Keenan Pearson and Maher, {L. James}",

note = "Publisher Copyright: {\textcopyright} 2023 Elsevier Ltd",

year = "2023",

month = aug,

day = "17",

doi = "10.1016/j.chembiol.2023.07.012",

language = "English (US)",

volume = "30",

pages = "855--857",

journal = "Cell Chemical Biology",

issn = "2451-9456",

publisher = "Elsevier Inc.",

number = "8",

}

TY - JOUR

T1 - A non-rational approach to optimized targeting of CRISPR-Cas9 complexes

AU - Wilbanks, Brandon

AU - Pearson, Keenan

AU - Maher, L. James

PY - 2023/8/17

Y1 - 2023/8/17

N2 - In this issue of Cell Chemical Biology, Bush et al.1 report an in vitro selection method for optimizing CRISPR-Cas9 single-guide RNAs. This approach may be useful in targeting previously intractable genomic sequences. The results also provide insights into which positions in single-guide RNAs are most amenable to modification.

AB - In this issue of Cell Chemical Biology, Bush et al.1 report an in vitro selection method for optimizing CRISPR-Cas9 single-guide RNAs. This approach may be useful in targeting previously intractable genomic sequences. The results also provide insights into which positions in single-guide RNAs are most amenable to modification.

UR - http://www.scopus.com/inward/record.url?scp=85167413899&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85167413899&partnerID=8YFLogxK

U2 - 10.1016/j.chembiol.2023.07.012

DO - 10.1016/j.chembiol.2023.07.012

M3 - Comment/debate

C2 - 37595547

AN - SCOPUS:85167413899

SN - 2451-9456

VL - 30

SP - 855

EP - 857

JO - Cell Chemical Biology

JF - Cell Chemical Biology

IS - 8

ER -

A non-rational approach to optimized targeting of CRISPR-Cas9 complexes

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this