A mouse model of familial oligoasthenoteratozoospermia

Subhash C. Juneja, Jan M. van Deursen

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Background: Hrb is an HIV-1 Rev-binding/interacting protein and is a cofactor for Rev export pathway. Hrb interacts with Eps15 homology (EH) domain-containing proteins and is a component of EH network and functions in vesicle sorting. Earlier, we reported that Hrb-deficient male mice are infertile and that they show oligozoospermia. Their sperm lack acrosomes and present globozoospermia. The aim of this study was: (i) to investigate the additional defects in spermatogenesis in Hrb-deficient mice; and (ii) to investigate the effect of acrosomelessness on spermatid differentiation in Hrb-deficient mice. Methods: Hrb-/- testes, epididymides, spermatids and sperm were analyzed by histology and electron microscopy. Centrioles were analyzed in spermatids and sperm by indirect immunofluorescence technique. Results: Hrb-/- male mice exhibited multiple anomalies during meiosis and spermiogenesis that produced developmentally impaired sperm with unshaped or deformed nuclei, loss in cell polarity, intracellular flagellar coiling, multinucleation, supernumerary centrioles and multiflagellation. A total of 13.0% Hrb-/- sperm showed macrocephaly. The Hrb-/- sperm exhibited variation in head size and shape, disarranged cellular organelles, nuclear and cytoplasmic vacuolization, mitochondrial loss or scattering and no forward motility. Conclusions: These aberrations, in Hrb-/- mouse spermatids and sperm, are reminiscent of human familial male infertility with oligoasthenoteratozoospermia syndrome. The Hrb-deficient mouse may be useful in understanding familial oligoasthenoteratozoospermia syndrome.

Original languageEnglish (US)
Pages (from-to)881-893
Number of pages13
JournalHuman Reproduction
Issue number4
StatePublished - Apr 2005


  • Centrioles
  • Manchette
  • Multiflagellation
  • Oligoasthenoteratozoospermia

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology


Dive into the research topics of 'A mouse model of familial oligoasthenoteratozoospermia'. Together they form a unique fingerprint.

Cite this