A mouse model for high-efficient Flp-recombinase-mediated genetic manipulation in the pancreas

Xiaohui Zhu, Jiaxiang Chen, Bin Wang, Lingxiang Wang, Jiale Wang, Dongfeng Feng, Yan Yang, Oliver Wang, Ashley N. Haddock, Ying Wang, Baoan Ji, Yan Bi

Research output: Contribution to journalArticlepeer-review


Background: Tissue and cell-specific gene targeting has been widely employed in biomedical research. In the pancreas, the commonly used Cre recombinase recognizes and recombines loxP sites. However, to selectively target different genes in distinct cells, a dual recombinase system is required. Method: We developed an alternative recombination system mediated by FLPo, which recognizes frt DNA sequences for pancreatic dual recombinase-mediated genetic manipulation. An IRES-FLPo cassette was targeted between the translation stop code and 3-UTR of the mouse pdx1 gene in a Bacterial Artificial Chromosome using recombineering technology. Transgenic BAC-Pdx1-FLPo mice were developed by pronuclear injection. Results: Highly efficient recombination activity was observed in the pancreas by crossing the founder mice with Flp reporter mice. When the BAC-Pdx1-FLPo mice were bred with conditional FSF-KRasG12D and p53 F/F mice, pancreatic cancer developed in the compound mice. The characteristics of pancreatic cancer resembled those derived from conditional LSL-KRasG12D and p53 L/L mice controlled by pdx1-Cre. Conclusions: We have generated a new transgenic mouse line expressing FLPo, which enables highly efficient pancreatic-specific gene recombination. When combined with other available Cre lines, this system can be utilized to target different genes in distinct cells for pancreatic research.

Original languageEnglish (US)
Pages (from-to)736-741
Number of pages6
Issue number6
StatePublished - Sep 2023


  • Animal models
  • Cancer
  • Dual recombinase
  • Pancreas
  • Pdx1-FlpO

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology


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