5-HT(2A) receptors stimulate mitogen-activated protein kinase via H2O2 generation in rat renal mesangial cells

Eddie L. Greene, Odette Houghton, Georgiann Collinsworth, Maria N. Garnovskaya, Toshio Nagai, Tahir Sajjad, Venugopala Bheemanathini, Jasjit S. Grewal, Richard V. Paul, John R. Raymond

Research output: Contribution to journalArticlepeer-review

76 Scopus citations


Serotonin (5-HT) stimulates mitogenesis in rat renal mesangial cells through a G protein-coupled 5-HT(2A) receptor. We tested the hypothesis that oxidants might be involved in the signal transduction pathway linking the receptor to extracellular signal-regulated protein kinase (ERK). 5-HT rapidly increased the activity and phosphorylation of ERK. These effects were blocked by the 5-HT(2A) receptor antagonist ketanserin. The peak effect was noted at 5-10 min, and half-maximal stimulation was achieved at 10-30 nM 5-HT. Chemical inhibitor and activator studies supported the involvement of phospholipase C, protein kinase C (PKC), and reactive oxygen species (ROS, i.e., H2O2 and superoxide) generated by an NAD(P)H oxidase-like enzyme in the ERK activation cascade. Mapping studies supported a location for the NAD(P)H oxidase enzyme and the ROS downstream from PKC. Our studies are most consistent with an ERK activation pathway as follows: 5-HT(2A) receptor → G(q) protein → phospholipase C → diacylglycerol → classical PKC → NAD(P)H oxidase → superoxide → superoxide dismutase → H2O2 → mitogen-activated extracellular signal-regulated kinase → ERK. These studies demonstrate a role for the 5-HT(2A) receptor in rapid, potent, and efficacious activation of ERK in rat renal mesangial cells. They support a role for oxidants in conveying the stimulatory signal from 5-HT, because 1) chemical antioxidants attenuate the 5-HT signal, 2) oxidants and 5-HT selectively activate ERK to a similar degree, 3) 5-HT produces superoxide and H2O2 in these cells, and 4) a specific enzyme [NAD(P)H oxidase] has been implicated as the source of the ROS, which react selectively downstream of classical PKC.

Original languageEnglish (US)
Pages (from-to)F650-F658
JournalAmerican Journal of Physiology - Renal Physiology
Issue number4 47-4
StatePublished - Apr 2000


  • Kidney
  • NADP(H) oxidase
  • Reactive oxygen species
  • Serotonin receptor
  • Signal transduction

ASJC Scopus subject areas

  • Physiology
  • Urology


Dive into the research topics of '5-HT(2A) receptors stimulate mitogen-activated protein kinase via H2O2 generation in rat renal mesangial cells'. Together they form a unique fingerprint.

Cite this