Tropomyosin Ser-283 pseudo-phosphorylation slows myofibril relaxation

Benjamin R. Nixon, Bin Liu, Beatrice Scellini, Chiara Tesi, Nicoletta Piroddi, Ozgur Ogut, R. John Solaro, Mark T. Ziolo, Paul M.L. Janssen, Jonathan P. Davis, Corrado Poggesi, Brandon J. Biesiadecki

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


Tropomyosin (Tm) is a central protein in the Ca2+ regulation of striated muscle. The αTm isoform undergoes phosphorylation at serine residue 283. While the biochemical and steady-state muscle function of muscle purified Tm phosphorylation have been explored, the effects of Tm phosphorylation on the dynamic properties of muscle contraction and relaxation are unknown. To investigate the kinetic regulatory role of αTm phosphorylation we expressed and purified native N-terminal acetylated Ser-283 wild-type, S283A phosphorylation null and S283D pseudo-phosphorylation Tm mutants in insect cells. Purified Tm's regulate thin filaments similar to that reported for muscle purified Tm. Steady-state Ca2+ binding to troponin C (TnC) in reconstituted thin filaments did not differ between the 3 Tm's, however disassociation of Ca2+ from filaments containing pseudo-phosphorylated Tm was slowed compared to wild-type Tm. Replacement of pseudo-phosphorylated Tm into myofibrils similarly prolonged the slow phase of relaxation and decreased the rate of the fast phase without altering activation kinetics. These data demonstrate that Tm pseudo-phosphorylation slows deactivation of the thin filament and muscle force relaxation dynamics in the absence of dynamic and steady-state effects on muscle activation. This supports a role for Tm as a key protein in the regulation of muscle relaxation dynamics.

Original languageEnglish (US)
Pages (from-to)30-38
Number of pages9
JournalArchives of Biochemistry and Biophysics
Issue number1
StatePublished - Jul 1 2013


  • Calcium
  • Human
  • Myofibril
  • Phosphorylation
  • Relaxation
  • Tropomyosin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology


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