@article{4ca76b25e13c4eba965269768a2bc988,
title = "Transcriptomic analysis of Streptococcus agalactiae periprosthetic joint infection",
abstract = "Although Streptococcus agalactiae periprosthetic joint infection (PJI) is not as prevalent as staphylococcal PJI, invasive S. agalactiae infection is not uncommon. Here, RNA-seq was used to perform transcriptomic analysis of S. agalactiae PJI using fluid derived from sonication of explanted arthroplasties of subjects with S. agalactiae PJI, with results compared to those of S. agalactiae strain NEM316 grown in vitro. A total of 227 genes with outlier expression were found (164 upregulated and 63 downregulated) between PJI sonicate fluid and in vitro conditions. Functional enrichment analysis showed genes involved in mobilome and inorganic ion transport and metabolism to be most enriched. Genes involved in nickel, copper, and zinc transport, were upregulated. Among known virulence factors, cyl operon genes, encoding β-hemolysin/cytolysin, were consistently highly expressed in PJI versus in vitro. The data presented provide insight into S. agalactiae PJI pathogenesis and may be a resource for identification of novel PJI therapeutics or vaccines against invasive S. agalactiae infections.",
author = "Cho, {Hye Kyung} and Thao Masters and Greenwood-Quaintance, {Kerryl E.} and Stephen Johnson and Jeraldo, {Patricio R.} and Nicholas Chia and Meng Pu and Abdel, {Matthew P.} and Robin Patel",
note = "Funding Information: Research reported in this publication was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health under award number R01AR056647. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Funding Information: Dr. Patel reports grants from ContraFect, TenNor Therapeutics Ltd, Hylomorph, BioFire, and Shionogi. Dr. Patel is a consultant to Curetis, Specific Technologies, Next Gen Diagnostics, PathoQuest, Selux Diagnostics, 1928 Diagnostics, PhAST, Torus, Mammoth Biosciences, and Qvella; monies are paid to Mayo Clinic. Dr. Patel is also a consultant to Netflix. In addition, Dr. Patel has a patent on PCR issued, a patent on an anti‐biofilm substance issued, and a patent on a device/method for sonication with royalties paid by Samsung to Mayo Clinic. Dr. Patel receives an editor's stipend from the Infectious Diseases Society of America, and honoraria from the National Board of Medical Examiners, the Infectious Diseases Board Review Course, and UpToDate Inc. All other authors declare no conflict of interests. Bordetella pertussis/parapertussis Publisher Copyright: {\textcopyright} 2021 Mayo Clinic. MicrobiologyOpen published by John Wiley & Sons Ltd.",
year = "2021",
month = nov,
doi = "10.1002/mbo3.1256",
language = "English (US)",
volume = "10",
journal = "MicrobiologyOpen",
issn = "2045-8827",
publisher = "John Wiley and Sons Inc.",
number = "6",
}