TY - JOUR
T1 - Transcription of histone H4, H3, and H1 cell cycle genes
T2 - Promoter factor HiNF-D contains CDC2, cyclin A, and an RB-related protein
AU - Van Wijnen, André J.
AU - Aziz, Farah
AU - Graña, Xavier
AU - De Luca, Antonio
AU - Desai, Rajesh K.
AU - Jaarsveld, Karen
AU - Last, Thomas J.
AU - Soprano, Kenneth
AU - Giordano, Antonio
AU - Lian, Jane B.
AU - Stein, Janet L.
AU - Stein, Gary S.
PY - 1994/12/20
Y1 - 1994/12/20
N2 - Cell cycle-controlled human histone genes are coordinately expressed during S phase, and transcriptional regulation involves a series of trans- acting factors (HiNFs). The proliferation-specific factor HiNF-D interacts with multiple recognition motifs in histone H4, H3, and H1 promoters. Using gel shift immunoassays, we show that CDC2, cyclin A, and an RB-related protein are ubiquitous subunits of HiNF-D binding activity isolated from several cell types. HiNF-D levels in vivo are sensitive to okadaic acid and staurosporine, indicating that HiNF-D activity and/or assembly is influenced by phosphorylation status. Thus, HiNF-D appears to be a multicomponent phosphoprotein that participates in coordinate control of multiple histone H4, H3, and H1 genes during the cell cycle. The presence of cell cycle mediators in the HiNF-D complex suggests linkage between transcriptional control of histones, enzymes involved in DNA synthesis, and the onset of DNA replication during the G1/S phase transition.
AB - Cell cycle-controlled human histone genes are coordinately expressed during S phase, and transcriptional regulation involves a series of trans- acting factors (HiNFs). The proliferation-specific factor HiNF-D interacts with multiple recognition motifs in histone H4, H3, and H1 promoters. Using gel shift immunoassays, we show that CDC2, cyclin A, and an RB-related protein are ubiquitous subunits of HiNF-D binding activity isolated from several cell types. HiNF-D levels in vivo are sensitive to okadaic acid and staurosporine, indicating that HiNF-D activity and/or assembly is influenced by phosphorylation status. Thus, HiNF-D appears to be a multicomponent phosphoprotein that participates in coordinate control of multiple histone H4, H3, and H1 genes during the cell cycle. The presence of cell cycle mediators in the HiNF-D complex suggests linkage between transcriptional control of histones, enzymes involved in DNA synthesis, and the onset of DNA replication during the G1/S phase transition.
KW - cell proliferation
KW - cyclin-dependent kinase
KW - gene expression
KW - protein phosphorylation
KW - tumor suppressor
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U2 - 10.1073/pnas.91.26.12882
DO - 10.1073/pnas.91.26.12882
M3 - Article
C2 - 7809140
AN - SCOPUS:0028558697
SN - 0027-8424
VL - 91
SP - 12882
EP - 12886
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 26
ER -