Tracing lymphopoiesis with the aid of a pTα-controlled reporter gene

Fotini Gounari, Iannis Aifantis, Colin Martin, Hans Joerg Fehling, Sonja Hoeflinger, Philip Leder, Harald von Boehmer, Boris Reizis

Research output: Contribution to journalArticlepeer-review


A transgenic reporter mouse strain, which expressed the human CD25 (hCD25) surface marker as a reporter under the control of the pre-T cell receptor α (pTα) promoter, was used to identify lymphoid precursors that expressed pTα intracellularly. The hCD25 reporter marked intra- and extrathymic precursors of lymphocytes but not myeloid cells. The earliest intrathymic precursors were the extrathymic precursors were common lymphoid progenitors (CLPs) that included CD19, B220+, Thyl+ and CD4+ cells. Thus, the pTα reporter can be used to trace lymphopoiesis between CLPs and αβ T cells. The slower extinction of the hCD25 reporter compared to pTα enabled us to define points at which pTα lineages branched off.

Original languageEnglish (US)
Pages (from-to)489-496
Number of pages8
JournalNature immunology
Issue number5
StatePublished - 2002

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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