The secretome of senescent preadipocytes influences the phenotype and function of cells of the vascular wall

Mojtaba Parvizi, Zachary C. Ryan, Sanam Ebtehaj, Bonnie K. Arendt, Ian R. Lanza

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Senescent cells accumulate in numerous tissues in several chronic conditions such as aging, obesity, and diabetes. These cells are in a state of irreversible cell-cycle arrest and secrete inflammatory cytokines, chemokines and other immune modulators that have paracrine effects on nearby tissues. Adipose tissue, in particular, harbors senescent cells, which have been linked with numerous chronic conditions and age-related comorbidities. Here we performed a series of in vitro experiments to determine the influence of senescent preadipocytes on key cell types found in vessel walls, including vascular smooth muscle cells (VSMCs), endothelial cells (ECs), macrophages (MQs), and adipose-derived stromal/stem cells (ASCs). Primary human preadipocytes were irradiated to trigger a senescence-like phenotype. VSMCs, ECs, MQs, and ASCs were exposed to conditioned media collected from irradiated preadipocytes or control preadipocytes. Additional experiments were performed where VSMCs, ECs, MQs, and ASCs were co-cultured with irradiated or control preadipocytes. The secretome of irradiated cells induced an inflammatory phenotype, decreased cell viability, disrupted proliferation and migration, and impaired metabolic function of these cell types in vitro. These maladaptive changes in response to senescent cell exposure provide early evidence in support of a hypothesis that senescent preadipocytes trigger phenotypic and functional changes in key cellular components of blood vessels that may contribute to vascular disease.

Original languageEnglish (US)
Article number165983
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Issue number1
StatePublished - Jan 1 2021


  • Endothelial cells
  • Inflammation
  • Macrophages
  • Senescence
  • Smooth muscle
  • Stem cells

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology


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