The reaction between nitric oxide and α-tocopheuol: A reappraisal

Neil Hogg, Ravinder Jit Singh, Steven P.A. Goss, B. Kalyanaraman

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Recently Gorbunov et al. reported that nitric oxide (·NO) can directly oxidize α-tocopherol to α-tocopheroxyl radical. We have reinvestigated this reaction and report that a direct reaction between ·NO and a-tocopherol does not occur. However, the reaction between ·NO and oxygen generates an oxidant which oxidizes α-tocopherol to α-tocopheryl quinone. Exposure of α-tocopherol to a low flux of ·NO generated from spermine NONOnte (100 μM) results in no consumption of α-tocopherol under either aerobic or anaerobic conditions. A higher flux of ·NO, generated from 1 mM spermine NONOate, oxidizes α-tocopherol only under aerobic conditions. Artifactual oxidation of α-tocopherol can be observed when using commercial ·NO that is contaminated with higher oxides of nitrogen, such as dinitrogen trioxide and dinitrogen tetraoxide.

Original languageEnglish (US)
Pages (from-to)696-702
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - Jul 25 1996

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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