The effects of polymorphisms on human gene targeting

David R. Deyle, Li B. Li, Gaoying Ren, David W. Russell

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


DNA mismatches that occur between vector homology arms and chromosomal target sequences reduce gene targeting frequencies in several species; however, this has not been reported in human cells. Here we demonstrate that even a single mismatched base pair can significantly decrease human gene targeting frequencies. In addition, we show that homology arm polymorphisms can be used to direct allele-specific targeting or to improve unfavorable vector designs that introduce deletions.

Original languageEnglish (US)
Pages (from-to)3119-3124
Number of pages6
JournalNucleic acids research
Issue number5
StatePublished - Mar 1 2014

ASJC Scopus subject areas

  • Genetics


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