TY - JOUR
T1 - The effects of acidic proteins and RNA on the histone inhibition of the DNA-dependent RNA synthesis in vitro
AU - Spelsberg, Thomas C.
AU - Hnilica, Lubomir S.
N1 - Funding Information:
The valuable technical assistance of Miss Sharon Tankersley is greatly appreciated. This investigation was supported by Grant CAo7746 from the U.S. Public
Funding Information:
Health Service, Grant E388 from the American Cancer Society, by The Robert A. Welch Foundation Grant GI38, and by the Institutional Grant IN-43-I-I.
PY - 1969/11/19
Y1 - 1969/11/19
N2 - 1. 1. Interactions of various polyanions with histones and the resulting effect of the DNA-dependent synthesis of RNA in vitro was investigated. None of the studied polyanions, nuclear acidic protein fractions from liver or thymus, RNA, polyribonucleotides and polyamino acids, were able to reverse the histone inhibition of RNA synthesis once the histone-DNA complex was established. 2. 2. However, the acidic nuclear proteins and poly amino acids, but not the RNA or polyribonucleotides, would prevent much of the histone inhibition of RNA synthesis in vitro if interacted directly with histones prior to their association with DNA. 3. 3. The failure of RNA and polyribonucleotides to reverse the inhibition of RNA synthesis in vitro by histones is due to the dissociation of RNA-histone complexes by the template DNA used in the RNA polymerase assay mixtures. A simple technique to measure histone-RNA-DNA complexes on millipore filters is described.
AB - 1. 1. Interactions of various polyanions with histones and the resulting effect of the DNA-dependent synthesis of RNA in vitro was investigated. None of the studied polyanions, nuclear acidic protein fractions from liver or thymus, RNA, polyribonucleotides and polyamino acids, were able to reverse the histone inhibition of RNA synthesis once the histone-DNA complex was established. 2. 2. However, the acidic nuclear proteins and poly amino acids, but not the RNA or polyribonucleotides, would prevent much of the histone inhibition of RNA synthesis in vitro if interacted directly with histones prior to their association with DNA. 3. 3. The failure of RNA and polyribonucleotides to reverse the inhibition of RNA synthesis in vitro by histones is due to the dissociation of RNA-histone complexes by the template DNA used in the RNA polymerase assay mixtures. A simple technique to measure histone-RNA-DNA complexes on millipore filters is described.
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U2 - 10.1016/0005-2787(69)90603-0
DO - 10.1016/0005-2787(69)90603-0
M3 - Article
C2 - 5357039
AN - SCOPUS:0014684440
SN - 0005-2787
VL - 195
SP - 63
EP - 75
JO - BBA Section Nucleic Acids And Protein Synthesis
JF - BBA Section Nucleic Acids And Protein Synthesis
IS - 1
ER -