Supercritical Carbon Dioxide–Based Sterilization of Decellularized Heart Valves

Ryan S. Hennessy, Soumen Jana, Brandon J. Tefft, Meghana R. Helder, Melissa D. Young, Rebecca R. Hennessy, Nicholas J. Stoyles, Amir Lerman

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


Sterilization of grafts is essential. Supercritical carbon dioxide, electrolyzed water, gamma radiation, ethanol-peracetic acid, and hydrogen peroxide techniques were compared for impact on sterility and mechanical integrity of porcine decellularized aortic valves. Ethanol-peracetic acid– and supercritical carbon dioxide–treated valves were found to be sterile using histology, microbe culture, and electron microscopy assays. The cusp tensile properties of supercritical carbon dioxide–treated valves were higher compared with valves treated with other techniques. Superior sterility and integrity was found in the decellularized valves treated with supercritical carbon dioxide sterilization. This sterilization technique may hold promise for other decellularized soft tissues.

Original languageEnglish (US)
Pages (from-to)71-84
Number of pages14
JournalJACC: Basic to Translational Science
Issue number1
StatePublished - 2017


  • decellularized
  • decontamination
  • heart valve
  • tensile properties
  • tissue engineering

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine


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