Staining with fluorescein diacetate correlates with hepatocyte function

Scott L. Nyberg, Russell A. Shatford, William D. Payne, Wei Shou Hu, Frank B. Cerra

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


To establish the importance of fluorescein diacetate (FDA) as a viability stain for cultured hepatocytes. we hypothesized that FDA staining would correlate positively with hepatocyte viability and function. Mixtures of live and dead cells were stained with FDA and scanned by flow cytometry. A close correlation was observed between the live cell fraction and percent viability as determined by FDA staining (R2 = 0.962). Hepatocytes were also sorted into low fluorescence and high fluorescence groups. Both albumin production and lidocaine metabolism (P-450 activity) were significantly increased in the high fluorescence group compared to the low fluorescence group. An automated, fluorescence-activated assay was useful for rapid assessment of hepatocyte viability. In addition. the intensity of green fluorescence following staining with FDA correlated well with two specific measures of hepatocyte function.

Original languageEnglish (US)
Pages (from-to)56-63
Number of pages8
JournalBiotechnic and Histochemistry
Issue number1
StatePublished - 1993


  • Albumin production
  • Flow cytometry
  • Fluorescein diacetate
  • Hepatocyte
  • Lidocaine metabolism. viability

ASJC Scopus subject areas

  • Histology
  • Medical Laboratory Technology


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