Soluble Interleukin-13Rα2 Decoy Receptor Inhibits Hodgkin's Lymphoma Growth in Vitro and in Vivo

Young Trieu, Xiao Yan Wen, Brian F. Skinnider, Mark R. Bray, Zhihua Li, Jaime O. Claudio, Esther Masih-Khan, Yuan Xiao Zhu, Suzanne Trudel, J. Andrea McCart, Tak W. Mak, A. Keith Stewart

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


Recent studies have demonstrated that the malignant Reed-Sternberg cells of Hodgkin's lymphoma (HL) secrete and are responsive to interleukin (IL)-13. We hypothesized that overexpression of a soluble IL-13 decoy receptor (sIL-13Rα2) via adenoviral-mediated gene transfer would inhibit IL-13-induced Reed-Sternberg cell proliferation. Western blot and ELISA analysis verified expression of sIL-13Rα2 in cell lysates and supernatants of AdsIL-13Rα2-transduced COS-7 cells. Treatment of two IL-13-responsive HL-derived cell lines, HDLM-2 and L-1236, with AdsIL-13Rα2-conditioned medium, resulted in the inhibition of cell proliferation, and down-regulated the phosphorylation of signal transducer and activator of transcription 6 (STAT6), an important mediator of IL-13 signaling. i.v. delivery of AdsIL-13Rα2 in NOD/SCID mice with s.c. implanted HDLM-2 cells delayed tumor onset and growth while enhancing survival compared with control mice. Intratumoral administration of AdsIL-13Rα2 led to the regression or stabilization of established tumors and was associated with diminished STAT6 phosphorylation. Our data demonstrate that AdsIL-13Rα2 can suppress HL growth in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)3271-3275
Number of pages5
JournalCancer research
Issue number9
StatePublished - May 1 2004

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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