Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling Couples Proliferation and Cell Death Mechanisms

Jesse D. Gelles; Jarvier N. Mohammed; Luis C. Santos; Diana Legarda; Adrian T. Ting; Jerry E. Chipuk

doi:10.1016/j.devcel.2019.08.016

Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling Couples Proliferation and Cell Death Mechanisms

Jesse D. Gelles
, Jarvier N. Mohammed
, Luis C. Santos
, Diana Legarda
, Adrian T. Ting
, Jerry E. Chipuk

Immunology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

To quantify cell death in high-throughput studies, Gelles et al. develop a robust method for single-cell and population-level analyses using real-time kinetic labeling (SPARKL). Example protocols and mathematical analyses detail the characterization of cell death kinetics and mechanisms, with coupled changes to proliferation, for use within high-volume comparative approaches.

Original language	English (US)
Pages (from-to)	277-291.e4
Journal	Developmental Cell
Volume	51
Issue number	2
DOIs	https://doi.org/10.1016/j.devcel.2019.08.016
State	Published - Oct 21 2019

Keywords

apoptosis
automated image analysis
cell death
ferroptosis
high-throughput
live-cell imaging
necroptosis
real-time assay

ASJC Scopus subject areas

Molecular Biology
General Biochemistry, Genetics and Molecular Biology
Developmental Biology
Cell Biology

Access to Document

10.1016/j.devcel.2019.08.016

Cite this

@article{1666cefe96e84929bd99ac60d8a04dff,

title = "Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling Couples Proliferation and Cell Death Mechanisms",

abstract = "To quantify cell death in high-throughput studies, Gelles et al. develop a robust method for single-cell and population-level analyses using real-time kinetic labeling (SPARKL). Example protocols and mathematical analyses detail the characterization of cell death kinetics and mechanisms, with coupled changes to proliferation, for use within high-volume comparative approaches.",

keywords = "apoptosis, automated image analysis, cell death, ferroptosis, high-throughput, live-cell imaging, necroptosis, real-time assay",

author = "Gelles, \{Jesse D.\} and Mohammed, \{Jarvier N.\} and Santos, \{Luis C.\} and Diana Legarda and Ting, \{Adrian T.\} and Chipuk, \{Jerry E.\}",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",

year = "2019",

month = oct,

day = "21",

doi = "10.1016/j.devcel.2019.08.016",

language = "English (US)",

volume = "51",

pages = "277--291.e4",

journal = "Developmental Cell",

issn = "1534-5807",

publisher = "Cell Press",

number = "2",

}

TY - JOUR

T1 - Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling Couples Proliferation and Cell Death Mechanisms

AU - Gelles, Jesse D.

AU - Mohammed, Jarvier N.

AU - Santos, Luis C.

AU - Legarda, Diana

AU - Ting, Adrian T.

AU - Chipuk, Jerry E.

PY - 2019/10/21

Y1 - 2019/10/21

N2 - To quantify cell death in high-throughput studies, Gelles et al. develop a robust method for single-cell and population-level analyses using real-time kinetic labeling (SPARKL). Example protocols and mathematical analyses detail the characterization of cell death kinetics and mechanisms, with coupled changes to proliferation, for use within high-volume comparative approaches.

AB - To quantify cell death in high-throughput studies, Gelles et al. develop a robust method for single-cell and population-level analyses using real-time kinetic labeling (SPARKL). Example protocols and mathematical analyses detail the characterization of cell death kinetics and mechanisms, with coupled changes to proliferation, for use within high-volume comparative approaches.

KW - apoptosis

KW - automated image analysis

KW - cell death

KW - ferroptosis

KW - high-throughput

KW - live-cell imaging

KW - necroptosis

KW - real-time assay

UR - https://www.scopus.com/pages/publications/85073221809

UR - https://www.scopus.com/pages/publications/85073221809#tab=citedBy

U2 - 10.1016/j.devcel.2019.08.016

DO - 10.1016/j.devcel.2019.08.016

M3 - Article

C2 - 31564612

AN - SCOPUS:85073221809

SN - 1534-5807

VL - 51

SP - 277-291.e4

JO - Developmental Cell

JF - Developmental Cell

IS - 2

ER -

Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling Couples Proliferation and Cell Death Mechanisms

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this