Redefining the properties of an osmotic agent in an intestinal-specific preservation solution

Kimberly Schlachter, Matthew S. Kokotilo, Jodi Carter, Aducio Thiesen, Angela Ochs, Rachel G. Khadaroo, Thomas A. Churchill

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


AIM: To investigate the effects of dextrans of various molecular weights (Mw) during a 12 h cold storage time-course on energetics, histology and mucosal infiltration of fluorescein isothiocyanate (FITC)-dextran. METHODS: Rodent intestines were isolated and received a standard University of Wisconsin vascular flush followed by intraluminal administration of a nutrientrich preservation solution containing dextrans of varying Mw: Group D1, 73 kdal; Group D2, 276 kdal; Group D3, 534 kdal; Group D4, 1185 kdal; Group D5, 2400 kdal. RESULTS: Using FITC-labeled dextrans, fluorescent micrographs demonstrated varying degrees of mucosal infiltration; lower Mw (groups D1-D3: 73-534 kdal) dextrans penetrated the mucosa as early as 2 h, whereas the largest dextran (D5: 2400 kdal) remained captive within the lumen and exhibited no permeability even after 12 h. After 12 h, median injury grades ranged from 6.5 to 7.5 in groups D1-D4 (73-1185 kdal) representing injury of the regenerative cryptal regions and submucosa; this was in contrast to group D5 (2400 kdal) which exhibited villus denudation (with intact crypts) corresponding to a median injury grade of 4 (P < 0.05). Analysis of tissue energetics reflected a strong positive correlation between Mw and adenosine triphosphate (r2 = 0.809), total adenylates (r2 = 0.865) and energy charge (r2 = 0.667). CONCLUSION: Our data indicate that dextrans of Mw > 2400 kdal act as true impermeant agents during 12 h ischemic storage when incorporated into an intraluminal preservation solution.

Original languageEnglish (US)
Pages (from-to)5701-5709
Number of pages9
JournalWorld journal of gastroenterology
Issue number45
StatePublished - Dec 2010


  • Cold storage
  • Intestinalspecific
  • Intraluminal preservation solution
  • Organ preservation
  • Osmotic impermeant

ASJC Scopus subject areas

  • Gastroenterology


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