@article{a4d65d710d564e47a6e43b14f616f753,
title = "Recommendations for the generation, quantification, storage, and handling of peptides used for mass spectrometry-based assays",
abstract = "BACKGROUND: For many years, basic and clinical researchers have taken advantage of the analytical sensitivity and specificity afforded by mass spectrometry in the measurement of proteins. Clinical laboratories are now beginning to deploy these work flows as well. For assays that use proteolysis to generate peptides for protein quantification and characterization, synthetic stable isotopelabeled internal standard peptides are of central importance. No general recommendations are currently available surrounding the use of peptides in protein mass spectrometric assays. CONTENT: The Clinical Proteomic Tumor Analysis Consortium of the National Cancer Institute has collaborated with clinical laboratorians, peptide manufacturers, metrologists, representatives of the pharmaceutical industry, and other professionals to develop a consensus set of recommendations for peptide procurement, characterization, storage, and handling, as well as approaches to the interpretation of the data generated by mass spectrometric protein assays. Additionally, the importance of carefully characterized reference materials-in particular, peptide standards for the improved concordance of amino acid analysis methods across the industry-is highlighted. The alignment of practices around the use of peptides and the transparency of sample preparation protocols should allow for the harmonization of peptide and protein quantification in research and clinical care.",
author = "Hoofnagle, {Andrew N.} and Whiteaker, {Jeffrey R.} and Carr, {Steven A.} and Eric Kuhn and Tao Liu and Massoni, {Sam A.} and Thomas, {Stefani N.} and {Reid Townsend}, R. and Zimmerman, {Lisa J.} and Emily Boja and Jing Chen and Crimmins, {Daniel L.} and Davies, {Sherri R.} and Yuqian Gao and Hiltke, {Tara R.} and Ketchum, {Karen A.} and Kinsinger, {Christopher R.} and Mehdi Mesri and Meyer, {Matthew R.} and Qian, {Wei Jun} and Schoenherr, {Regine M.} and Scott, {Mitchell G.} and Tujin Shi and Whiteley, {Gordon R.} and Wrobel, {John A.} and Chaochao Wu and Ackermann, {Brad L.} and Ruedi Aebersold and Barnidge, {David R.} and Bunk, {David M.} and Nigel Clarke and Fishman, {Jordan B.} and Grant, {Russ P.} and Ulrike Kusebauch and Kushnir, {Mark M.} and Lowenthal, {Mark S.} and Moritz, {Robert L.} and Hendrik Neubert and Patterson, {Scott D.} and Rockwood, {Alan L.} and John Rogers and Singh, {Ravinder J.} and {Van Eyk}, {Jennifer E.} and Wong, {Steven H.} and Shucha Zhang and Chan, {Daniel W.} and Xian Chen and Ellis, {Matthew J.} and Liebler, {Daniel C.} and Rodland, {Karin D.} and Henry Rodriguez and Smith, {Richard D.} and Zhen Zhang and Hui Zhang and Paulovich, {Amanda G.}",
note = "Funding Information: A.N. Hoofnagle, Waters Inc. and Thermo Inc.; J.E. Van Eyk, NIDDK (U01DK085689); D.W. Chan, NCI (U24CA115102); R.L. Moritz, NIGMS (P50GM076547 and R01GM087221); S.A. Carr (PI), A.N. Hoofnagle, E. Kuhn, A.G. Paulovich (PI), and R.M. Schoenherr, NCI CPTAC (U24CA160034); Y. Gao, T. Liu, W-J. Qian, K.D. Rodland (PI), T. Shi, R.D. Smith (PI), and C. Wu, NCI CPTAC (U24CA160019); D.W. Chan (PI), J. Chen, S.N. Thomas, H. Zhang (PI), and Z. Zhang (PI), NCI CPTAC (U24CA160036); X. Chen (PI), D.L. Crimmins, S.R. Davies, M.J. Ellis (PI), M.R. Meyer, M.G. Scott, and R.R. Townsend (PI), NCI CPTAC (U24CA160035); D.C. Liebler (PI) and L.J. Zimmerman, NCI CPTAC (U24CA159988). Publisher Copyright: {\textcopyright} 2015 American Association for Clinical Chemistry.",
year = "2016",
month = jan,
doi = "10.1373/clinchem.2015.250563",
language = "English (US)",
volume = "62",
pages = "48--69",
journal = "Clinical chemistry",
issn = "0009-9147",
publisher = "American Association for Clinical Chemistry Inc.",
number = "1",
}