@inbook{28ec54e9046b45e4bd5ed5b7a38f4959,
title = "Quantification of RNA content in reconstituted ebola virus nucleocapsids by immunoprecipitation",
abstract = "Immunoprecipitations are commonly used to isolate proteins or protein complexes and assess protein-protein interactions; however, they can also be used to assess protein-RNA complexes. Here we describe an adapted RNA immunoprecipitation technique that permits the quantification of RNA content in Ebola virus nucleocapsids that have been reconstituted in vitro by transient transfection.",
keywords = "Ebola virus, Immunoprecipitation, Nucleocapsid, Nucleoprotein, RNA, VP24, VP35, qRT-PCR",
author = "Logan Banadyga and Hideki Ebihara",
note = "Funding Information: The authors are grateful to Brandi Williamson for her critical review of this manuscript. The authors are supported by the Division of Intramural Research, NIAID, NIH. Opinions, interpretations, conclusions, and recommendations are those of the authors and are not necessarily endorsed by the NIH Publisher Copyright: {\textcopyright} 2017, Springer Science+Business Media LLC.",
year = "2017",
doi = "10.1007/978-1-4939-7116-9_7",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "93--107",
booktitle = "Methods in Molecular Biology",
}