Purification and Characterization of a Novel Growth Factor from Human Breast Cancer Cells

Ruth Lupu, Anton Wellstein, James Sheridan, Bruce W. Ennis, Gerhard Zugmaier, Deborah Katz, Marc E. Lippman, Robert B. Dickson

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


We have purified and characterized a novel 30-kDa glycoprotein (gp30) with TGF±-like properties secreted from the estrogen receptor negative breast cancer cell line MDA-MB-231. This factor was immunoprecipitated by an anti-TGF± polyclonal antibody and also had TGF±-like biological activity, as assayed by EGF radioreceptor assay and anchorage-independent assays. In addition, the novel growth factor stimulated phosphorylation of the EGF receptor and erbB-2 receptor. However, the novel growth factor, unlike EGF and TGF±, bound to heparin-Sepharose. Purification of gp30 was obtained to apparent homogeneity by heparin affinity chromatography and subsequent reversed-phase chromatography. Tunicamycin treatment in vivo or N-glycanase deglycosylation in vitro revealed a putative precursor of approximately 22 kDa molecular mass in contrast to the“normal”16-kDa precursor species for TGF±. In vitro translation of total mRNA from MDA-MB-231 cells confirmed the size of the putative precursor. Biochemical characterization of gp30 was begun by V8 protease digestion of the deglycosylated polypeptide and the translated products. Peptide mapping of V8-digested, immunoprecipitated material suggests that the amino acid sequence of this unique protein is distinct from mature TGF± and not the result of a posttranslational modification of the precursor. We conclude that this TGF±-like (gp30) polypeptide is a novel growth factor with agonistic activity for both EGF and erbB-2 receptors.

Original languageEnglish (US)
Pages (from-to)7330-7340
Number of pages11
Issue number32
StatePublished - Feb 1 1992

ASJC Scopus subject areas

  • Biochemistry


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