Partial purification by vacuum distillation of a factor that increases the junctional acetylcholinesterase of organ cultured rat diaphragm

S. G. Younkin; L. H. Younkin; M. Zoller; C. Rosenstein

doi:10.1016/0006-8993(82)90188-3

Partial purification by vacuum distillation of a factor that increases the junctional acetylcholinesterase of organ cultured rat diaphragm

S. G. Younkin, L. H. Younkin, M. Zoller, C. Rosenstein

Neuroscience

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Organ cultured rat diaphragm segments were used to assay a factor which increases endplate-specific acetylcholinesterase. This factor can be substantially purified by vacuum distilling it at 37°C into a dry-ice-acetone cold trap. Sources from which the factor can be distilled include eel electric organ homogenate, rat diaphragm homogenate, and medium conditioned with neurally stimulated muscle.

Original language	English (US)
Pages (from-to)	192-194
Number of pages	3
Journal	Brain Research
Volume	249
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(82)90188-3
State	Published - Oct 7 1982

Keywords

acetylcholinesterase
neuromuscular junction
organ culture
rat diaphragm
trophic factor

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(82)90188-3

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title = "Partial purification by vacuum distillation of a factor that increases the junctional acetylcholinesterase of organ cultured rat diaphragm",

abstract = "Organ cultured rat diaphragm segments were used to assay a factor which increases endplate-specific acetylcholinesterase. This factor can be substantially purified by vacuum distilling it at 37°C into a dry-ice-acetone cold trap. Sources from which the factor can be distilled include eel electric organ homogenate, rat diaphragm homogenate, and medium conditioned with neurally stimulated muscle.",

keywords = "acetylcholinesterase, neuromuscular junction, organ culture, rat diaphragm, trophic factor",

author = "Younkin, {S. G.} and Younkin, {L. H.} and M. Zoller and C. Rosenstein",

note = "Funding Information: This investigation was supported by N.I.H. Grant NS-15219 and by a grant from the Muscular Dystrophy Association.",

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AU - Younkin, S. G.

AU - Younkin, L. H.

AU - Zoller, M.

AU - Rosenstein, C.

N1 - Funding Information: This investigation was supported by N.I.H. Grant NS-15219 and by a grant from the Muscular Dystrophy Association.

PY - 1982/10/7

Y1 - 1982/10/7

N2 - Organ cultured rat diaphragm segments were used to assay a factor which increases endplate-specific acetylcholinesterase. This factor can be substantially purified by vacuum distilling it at 37°C into a dry-ice-acetone cold trap. Sources from which the factor can be distilled include eel electric organ homogenate, rat diaphragm homogenate, and medium conditioned with neurally stimulated muscle.

AB - Organ cultured rat diaphragm segments were used to assay a factor which increases endplate-specific acetylcholinesterase. This factor can be substantially purified by vacuum distilling it at 37°C into a dry-ice-acetone cold trap. Sources from which the factor can be distilled include eel electric organ homogenate, rat diaphragm homogenate, and medium conditioned with neurally stimulated muscle.

KW - acetylcholinesterase

KW - neuromuscular junction

KW - organ culture

KW - rat diaphragm

KW - trophic factor

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Partial purification by vacuum distillation of a factor that increases the junctional acetylcholinesterase of organ cultured rat diaphragm

Abstract

Keywords

ASJC Scopus subject areas

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