Paracrine mitogenic effect of human endothelial progenitor cells: Role of interleukin-8

Tongrong He, Timothy E. Peterson, Zvonimir S. Katusic

Research output: Contribution to journalArticlepeer-review

78 Scopus citations


Endothelial progenitor cells (EPCs) play an important role in repair of vascular injury and neovascularization. Molecular mechanisms underlying vascular effects of EPCs are not fully understood. The present study was designed to test the hypothesis that human EPCs exert a strong paracrine mitogenic effect on mature endothelial cells. Levels of interleukin-8 (IL-8) were significantly higher in conditioned medium (CM) collected from EPCs than in CM derived from mature endothelial cells [umbilical vein endothelial cells (HUVECs) and coronary artery endothelial cells (CAECs)]. CM of EPCs stimulated proliferation of HUVECs and CAECs. This mitogenic effect was partially inhibited by IL-8-neutralizing antibody. In contrast, CM of HUVECs and CAECs had a weak or no mitogenic effect on mature endothelial cells. Our results demonstrate significantly higher levels of IL-8 secretion by human EPCs than by mature endothelial cells. IL-8 appears to be an important mediator of the paracrine mitogenic effect of EPCs.

Original languageEnglish (US)
Pages (from-to)H968-H972
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number2 58-2
StatePublished - Aug 2005


  • Angiogenesis
  • Cytokines
  • Neovascularization

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


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