Natriuretic peptide infusion reduces myocardial injury during acute ischemia/reperfusion

Birgitte S. Kousholt, Jens K.Rolighed Larsen, Line Bisgaard, John C. Burnett, John Michael Hasenkam, Jens P. Goetze

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Aim The aim of this study was to determine whether a natriuretic peptide infusion during reperfusion can reduce cardiomyocyte ischemia-reperfusion damage. Materials and methods The effect of B-type natriuretic peptide (BNP) activity was assessed in vitro and in vivo: the cellular effect was determined by assessment of intracellular caspase activity and troponin T release from cultured HL-1 cells subjected to short-term hypoxia-reperfusion. Cardiac effects were further examined in pigs (n =25) that had been subjected to 1 h of regional cardiac ischemia, followed by 3 h of reperfusion. Results HL-1 cardiomyocytes responded to exogenous BNP with increased cGMP activity (~3-fold, P = 0.0037) and hypoxia-reperfusion with increased vascular endothelial growth factor and BNPmRNA contents (2.3- and 2.5-fold, respectively, P < 0.0001) and caspase activity (2.9-fold, P = 0.03), but without a decrease in apoptotic changes in the BNP-stimulated cells. Pigs tolerated the BNP and CD-NP (a CNP analogue) infusion well, with a decrease in systemic blood pressure (~15 mmHg) and increased diuresis compared with the controls. Left ventricular pressure decreased in the pigs that received BNP infusion compared with controls (P= 0.02). A similar trend was observed in the pigs that received CD-NP infusion, although this was not significant (P = 0.3). BNP and CD-NP infusion in pigs reduced total cardiac troponin T release by 46 and 40%, respectively (P = 0.0015 and 0.0019), and were associated with improved RNA integrity in the ischemic left ventricular region (P < 0.05). Conclusion We report that natriuretic peptide infusion in vivo reduces cardiomyocyte injury in acute ischemia-reperfusion, possibly through indirect mechanisms (e.g. increased diuresis and vasodilation). The results suggest a role for natriuretic peptide therapy in human cardiac ischemia. Cardiovasc Endocrinol 1:4-12

Original languageEnglish (US)
Pages (from-to)4-12
Number of pages9
JournalCardiovascular Endocrinology
Issue number1
StatePublished - Mar 2012


  • BNP
  • CD-NP
  • Ischemia-reperfusion injury
  • Myocardial infarction
  • Porcine

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrine and Autonomic Systems
  • Cardiology and Cardiovascular Medicine


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