Multiplication stimulating activity regulates ornithine decarboxylase in isolated porcine granutlosa cells in vitro

Johannes D. Veldhuis; James M. Hammond

doi:10.1080/07435807909061109

Multiplication stimulating activity regulates ornithine decarboxylase in isolated porcine granutlosa cells in vitro

Johannes D. Veldhuis, James M. Hammond

Endocrinology, Diabetes, Metabolism, and Nutrition

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The polypeptide growth factor, multiplication stimulating activity (MSA), stimulates ornithine decarboxylase (ODC) activity in isolated porcine granulosa cells maintained under chemically defined conditions in vitro. The stimulatory action of MSA is saturable, and dose-dependent (0.1-1000 ng/ml). MSA effects are additive to those of luteinizing hormone (LH), but not those of follicle stimulating hormone (FSH). Stimulation of ODC activity by MSA requires cellular protein and RNA synthesis, and appears to be mediated independently of cyclic AMP. These observations provide the first demonstration of MSA action in the mammalian ovary.

Original language	English (US)
Pages (from-to)	299-309
Number of pages	11
Journal	Endocrine Research
Volume	6
Issue number	4
DOIs	https://doi.org/10.1080/07435807909061109
State	Published - 1979

ASJC Scopus subject areas

Endocrinology

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10.1080/07435807909061109

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title = "Multiplication stimulating activity regulates ornithine decarboxylase in isolated porcine granutlosa cells in vitro",

abstract = "The polypeptide growth factor, multiplication stimulating activity (MSA), stimulates ornithine decarboxylase (ODC) activity in isolated porcine granulosa cells maintained under chemically defined conditions in vitro. The stimulatory action of MSA is saturable, and dose-dependent (0.1-1000 ng/ml). MSA effects are additive to those of luteinizing hormone (LH), but not those of follicle stimulating hormone (FSH). Stimulation of ODC activity by MSA requires cellular protein and RNA synthesis, and appears to be mediated independently of cyclic AMP. These observations provide the first demonstration of MSA action in the mammalian ovary.",

author = "Veldhuis, {Johannes D.} and Hammond, {James M.}",

note = "Funding Information: We thank the Shamokin Packing Corp. for provision of porcine ovaries, Patricia Klase for meticulous technical assistance, and Marlene Thompson for excellent support in the preparation of this manuscript. This work was supported by NIH Grant No. HD10122 and by a National Research Service Award, No. lF32HD05755 from the NICHD to Dr. J.D. Veldhuis. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1979",

doi = "10.1080/07435807909061109",

language = "English (US)",

volume = "6",

pages = "299--309",

journal = "Endocrine Research",

issn = "0743-5800",

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T1 - Multiplication stimulating activity regulates ornithine decarboxylase in isolated porcine granutlosa cells in vitro

AU - Veldhuis, Johannes D.

AU - Hammond, James M.

N1 - Funding Information: We thank the Shamokin Packing Corp. for provision of porcine ovaries, Patricia Klase for meticulous technical assistance, and Marlene Thompson for excellent support in the preparation of this manuscript. This work was supported by NIH Grant No. HD10122 and by a National Research Service Award, No. lF32HD05755 from the NICHD to Dr. J.D. Veldhuis. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1979

Y1 - 1979

N2 - The polypeptide growth factor, multiplication stimulating activity (MSA), stimulates ornithine decarboxylase (ODC) activity in isolated porcine granulosa cells maintained under chemically defined conditions in vitro. The stimulatory action of MSA is saturable, and dose-dependent (0.1-1000 ng/ml). MSA effects are additive to those of luteinizing hormone (LH), but not those of follicle stimulating hormone (FSH). Stimulation of ODC activity by MSA requires cellular protein and RNA synthesis, and appears to be mediated independently of cyclic AMP. These observations provide the first demonstration of MSA action in the mammalian ovary.

AB - The polypeptide growth factor, multiplication stimulating activity (MSA), stimulates ornithine decarboxylase (ODC) activity in isolated porcine granulosa cells maintained under chemically defined conditions in vitro. The stimulatory action of MSA is saturable, and dose-dependent (0.1-1000 ng/ml). MSA effects are additive to those of luteinizing hormone (LH), but not those of follicle stimulating hormone (FSH). Stimulation of ODC activity by MSA requires cellular protein and RNA synthesis, and appears to be mediated independently of cyclic AMP. These observations provide the first demonstration of MSA action in the mammalian ovary.

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JO - Endocrine Research

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Multiplication stimulating activity regulates ornithine decarboxylase in isolated porcine granutlosa cells in vitro

Abstract

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