MUC1 epithelial mucin (CD227) is expressed by activated dendritic cells

M. Wykes; K. P.A. MacDonald; M. Tran; R. J. Quin; P. X. Xing; S. J. Gendler; D. N.J. Hart; Michael A. McGuckin

MUC1 epithelial mucin (CD227) is expressed by activated dendritic cells

M. Wykes, K. P.A. MacDonald, M. Tran, R. J. Quin, P. X. Xing, S. J. Gendler, D. N.J. Hart, Michael A. McGuckin

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

The MUC1 mucin (CD227) is a cell surface mucin originally thought to be restricted to epithelial tissues. We report that CD227 is expressed on human blood dendritic cells (DC) and monocyte-derived DC following in vitro activation. Freshly isolated murine splenic DC had very low levels of CD227; however, all DC expressed CD227 following in vitro culture. In the mouse spleen, CD227 was seen on clusters within the red pulp and surrounding the marginal zone in the white pulp. Additionally, we confirm CD227 expression by activated human T cells and show for the first time that the CD227 cytoplasmic domain is tyrosine-phosphorylated in activated T cells and DC and is associated with other phosphoproteins, indicating a role in signaling. The function of CD227 on DC and T cells requires further elucidation.

Original language	English (US)
Pages (from-to)	692-701
Number of pages	10
Journal	Journal of Leukocyte Biology
Volume	72
Issue number	4
State	Published - Oct 1 2002

Keywords

Activation
Glycoprotein
Sialomucin
T cell

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Cell Biology

Cite this

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title = "MUC1 epithelial mucin (CD227) is expressed by activated dendritic cells",

abstract = "The MUC1 mucin (CD227) is a cell surface mucin originally thought to be restricted to epithelial tissues. We report that CD227 is expressed on human blood dendritic cells (DC) and monocyte-derived DC following in vitro activation. Freshly isolated murine splenic DC had very low levels of CD227; however, all DC expressed CD227 following in vitro culture. In the mouse spleen, CD227 was seen on clusters within the red pulp and surrounding the marginal zone in the white pulp. Additionally, we confirm CD227 expression by activated human T cells and show for the first time that the CD227 cytoplasmic domain is tyrosine-phosphorylated in activated T cells and DC and is associated with other phosphoproteins, indicating a role in signaling. The function of CD227 on DC and T cells requires further elucidation.",

keywords = "Activation, Glycoprotein, Sialomucin, T cell",

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T1 - MUC1 epithelial mucin (CD227) is expressed by activated dendritic cells

AU - Wykes, M.

AU - MacDonald, K. P.A.

AU - Tran, M.

AU - Quin, R. J.

AU - Xing, P. X.

AU - Gendler, S. J.

AU - Hart, D. N.J.

AU - McGuckin, Michael A.

PY - 2002/10/1

Y1 - 2002/10/1

N2 - The MUC1 mucin (CD227) is a cell surface mucin originally thought to be restricted to epithelial tissues. We report that CD227 is expressed on human blood dendritic cells (DC) and monocyte-derived DC following in vitro activation. Freshly isolated murine splenic DC had very low levels of CD227; however, all DC expressed CD227 following in vitro culture. In the mouse spleen, CD227 was seen on clusters within the red pulp and surrounding the marginal zone in the white pulp. Additionally, we confirm CD227 expression by activated human T cells and show for the first time that the CD227 cytoplasmic domain is tyrosine-phosphorylated in activated T cells and DC and is associated with other phosphoproteins, indicating a role in signaling. The function of CD227 on DC and T cells requires further elucidation.

AB - The MUC1 mucin (CD227) is a cell surface mucin originally thought to be restricted to epithelial tissues. We report that CD227 is expressed on human blood dendritic cells (DC) and monocyte-derived DC following in vitro activation. Freshly isolated murine splenic DC had very low levels of CD227; however, all DC expressed CD227 following in vitro culture. In the mouse spleen, CD227 was seen on clusters within the red pulp and surrounding the marginal zone in the white pulp. Additionally, we confirm CD227 expression by activated human T cells and show for the first time that the CD227 cytoplasmic domain is tyrosine-phosphorylated in activated T cells and DC and is associated with other phosphoproteins, indicating a role in signaling. The function of CD227 on DC and T cells requires further elucidation.

KW - Activation

KW - Glycoprotein

KW - Sialomucin

KW - T cell

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MUC1 epithelial mucin (CD227) is expressed by activated dendritic cells

Abstract

Keywords

ASJC Scopus subject areas

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