@article{c634bd42b4804a0084a87b34d4b038b6,
title = "Molecular pathology of total knee arthroplasty instability defined by RNA-seq",
abstract = "Total knee arthroplasty (TKA) is a durable and reliable procedure to alleviate pain and improve joint function. However, failures related to flexion instability sometimes occur. The goal of this study was to define biological differences between tissues from patients with and without flexion instability of the knee after TKA. Human knee joint capsule tissues were collected at the time of primary or revision TKAs and analyzed by RT-qPCR and RNA-seq, revealing novel patterns of differential gene expression between the two groups. Interestingly, genes related to collagen production and extracellular matrix (ECM) degradation were higher in samples from patients with flexion instability. Partitioned clustering analyses further emphasized differential gene expression patterns between sample types that may help guide clinical interpretations of this complication. Future efforts to disentangle the effects of physical and biological (e.g., transcriptomic modifications) risk factors will aid in further characterizing and avoiding flexion instability after TKA.",
keywords = "Cell biology, Flexion instability, Molecular genetics, Revision total knee arthroplasty, Total knee arthroplasty",
author = "Lewallen, {Eric A.} and Salib, {Christopher G.} and Trousdale, {William H.} and Berry, {Charlotte E.} and Hanssen, {Gabrielle M.} and Robin, {Joseph X.} and Tibbo, {Meagan E.} and Anthony Viste and Nicolas Reina and Morrey, {Mark E.} and Joaquin Sanchez-Sotelo and Hanssen, {Arlen D.} and Berry, {Daniel J.} and {van Wijnen}, {Andre J.} and Abdel, {Matthew P.}",
note = "Funding Information: We thank members of the Abdel and van Wijnen laboratories, and in particular Scott M. Riester, M.D., for stimulating discussions and/or assistance with reagents and procedures. Research reported in this publication was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health under award numbers R01 AR049069 (to AJVW) and F32 AR068154 (to EAL). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. We also appreciate the generous philanthropic support of William H. and Karen J. Eby, as well as the Anna-Maria and Stephen Kellen Foundation, and the charitable foundations in their names. Matthew P. Abdel, Arlen D. Hanssen, and Daniel J. Berry receive royalties for hip and knee related implants. No other authors have conflicts of interest to disclosure. Funding Information: We thank members of the Abdel and van Wijnen laboratories, and in particular Scott M. Riester, M.D., for stimulating discussions and/or assistance with reagents and procedures. Research reported in this publication was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health under award numbers R01 AR049069 (to AJVW) and F32 AR068154 (to EAL). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. We also appreciate the generous philanthropic support of William H. and Karen J. Eby, as well as the Anna-Maria and Stephen Kellen Foundation, and the charitable foundations in their names. Matthew P. Abdel, Arlen D. Hanssen, and Daniel J. Berry receive royalties for hip and knee related implants. No other authors have conflicts of interest to disclosure. Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",
year = "2018",
month = sep,
doi = "10.1016/j.ygeno.2017.11.001",
language = "English (US)",
volume = "110",
pages = "247--256",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "5",
}