Molecular cloning and sequencing of the cDNA encoding the feline FcγRIIIA (CD16) homologue

Yorihiro Nishimura; Takayuki Miyazawa; Yasuhiro Ikeda; Yoshihiro Izumiya; Kazuya Nakamura; Eiji Sato; Takeshi Mikami; Eiji Takahashi

doi:10.1016/S0165-2427(00)00156-2

Molecular cloning and sequencing of the cDNA encoding the feline FcγRIIIA (CD16) homologue

Yorihiro Nishimura, Takayuki Miyazawa, Yasuhiro Ikeda, Yoshihiro Izumiya, Kazuya Nakamura, Eiji Sato, Takeshi Mikami, Eiji Takahashi

Molecular Medicine

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We amplified the cDNA encoding the feline FcγRIIIA (CD16) homologue from peripheral blood mononuclear cells by polymerase chain reaction and cloned two forms of FCGR3A cDNA. Sequencing analysis revealed that the open reading frame of feline FCGR3A cDNA consists of 750 or 747 base pairs encoding 250 or 249 amino acid residues, respectively. Comparison of the predicted amino acid sequence of feline FCGR3A cDNA with those of other mammalians' homologues revealed that the extracellular domain has a relatively low homology. However, the cytoplasmic domain contained an 8-amino acid motif, Leu-Phe-Val-Val-Asp-Thr-Gly-Leu, which was considered to interact with an accessory molecule such as the γ chain of Fc receptors for IgE to form heterodimeric complexes. Copyright (C) 2000 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	353-359
Number of pages	7
Journal	Veterinary Immunology and Immunopathology
Volume	73
Issue number	3-4
DOIs	https://doi.org/10.1016/S0165-2427(00)00156-2
State	Published - Mar 15 2000

Keywords

CD16
Cat
Cloning
FcγRIII
cDNA

ASJC Scopus subject areas

Immunology
veterinary(all)

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10.1016/S0165-2427(00)00156-2

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title = "Molecular cloning and sequencing of the cDNA encoding the feline FcγRIIIA (CD16) homologue",

abstract = "We amplified the cDNA encoding the feline FcγRIIIA (CD16) homologue from peripheral blood mononuclear cells by polymerase chain reaction and cloned two forms of FCGR3A cDNA. Sequencing analysis revealed that the open reading frame of feline FCGR3A cDNA consists of 750 or 747 base pairs encoding 250 or 249 amino acid residues, respectively. Comparison of the predicted amino acid sequence of feline FCGR3A cDNA with those of other mammalians' homologues revealed that the extracellular domain has a relatively low homology. However, the cytoplasmic domain contained an 8-amino acid motif, Leu-Phe-Val-Val-Asp-Thr-Gly-Leu, which was considered to interact with an accessory molecule such as the γ chain of Fc receptors for IgE to form heterodimeric complexes. Copyright (C) 2000 Elsevier Science B.V.",

keywords = "CD16, Cat, Cloning, FcγRIII, cDNA",

author = "Yorihiro Nishimura and Takayuki Miyazawa and Yasuhiro Ikeda and Yoshihiro Izumiya and Kazuya Nakamura and Eiji Sato and Takeshi Mikami and Eiji Takahashi",

note = "Funding Information: The authors thank Dr. M. Hattori (Kyoto University, Kyoto, Japan) for providing recombinant human interleukin-2-producing Ltk - IL-2.23 cells. This study was supported in part by grants from the Ministry of Education, Science, Sports and Culture of Japan. Y. Nishimura, Y. Ikeda, Y. Izumiya, and E. Sato are supported by Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists.",

year = "2000",

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doi = "10.1016/S0165-2427(00)00156-2",

language = "English (US)",

volume = "73",

pages = "353--359",

journal = "Veterinary Immunology and Immunopathology",

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T1 - Molecular cloning and sequencing of the cDNA encoding the feline FcγRIIIA (CD16) homologue

AU - Nishimura, Yorihiro

AU - Miyazawa, Takayuki

AU - Ikeda, Yasuhiro

AU - Izumiya, Yoshihiro

AU - Nakamura, Kazuya

AU - Sato, Eiji

AU - Mikami, Takeshi

AU - Takahashi, Eiji

N1 - Funding Information: The authors thank Dr. M. Hattori (Kyoto University, Kyoto, Japan) for providing recombinant human interleukin-2-producing Ltk - IL-2.23 cells. This study was supported in part by grants from the Ministry of Education, Science, Sports and Culture of Japan. Y. Nishimura, Y. Ikeda, Y. Izumiya, and E. Sato are supported by Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists.

PY - 2000/3/15

Y1 - 2000/3/15

N2 - We amplified the cDNA encoding the feline FcγRIIIA (CD16) homologue from peripheral blood mononuclear cells by polymerase chain reaction and cloned two forms of FCGR3A cDNA. Sequencing analysis revealed that the open reading frame of feline FCGR3A cDNA consists of 750 or 747 base pairs encoding 250 or 249 amino acid residues, respectively. Comparison of the predicted amino acid sequence of feline FCGR3A cDNA with those of other mammalians' homologues revealed that the extracellular domain has a relatively low homology. However, the cytoplasmic domain contained an 8-amino acid motif, Leu-Phe-Val-Val-Asp-Thr-Gly-Leu, which was considered to interact with an accessory molecule such as the γ chain of Fc receptors for IgE to form heterodimeric complexes. Copyright (C) 2000 Elsevier Science B.V.

AB - We amplified the cDNA encoding the feline FcγRIIIA (CD16) homologue from peripheral blood mononuclear cells by polymerase chain reaction and cloned two forms of FCGR3A cDNA. Sequencing analysis revealed that the open reading frame of feline FCGR3A cDNA consists of 750 or 747 base pairs encoding 250 or 249 amino acid residues, respectively. Comparison of the predicted amino acid sequence of feline FCGR3A cDNA with those of other mammalians' homologues revealed that the extracellular domain has a relatively low homology. However, the cytoplasmic domain contained an 8-amino acid motif, Leu-Phe-Val-Val-Asp-Thr-Gly-Leu, which was considered to interact with an accessory molecule such as the γ chain of Fc receptors for IgE to form heterodimeric complexes. Copyright (C) 2000 Elsevier Science B.V.

KW - CD16

KW - Cat

KW - Cloning

KW - FcγRIII

KW - cDNA

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JO - Veterinary Immunology and Immunopathology

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ER -

Molecular cloning and sequencing of the cDNA encoding the feline FcγRIIIA (CD16) homologue

Abstract

Keywords

ASJC Scopus subject areas

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