Molecular Cloning and Functional Expression of the Human Gallbladder Cholecystokinin A Receptor

Charles D. Ulrich; Irene Ferber; Eileen Holicky; Elizabeth Hadac; Gary Buell; Laurence J. Miller

doi:10.1006/bbrc.1993.1610

Molecular Cloning and Functional Expression of the Human Gallbladder Cholecystokinin A Receptor

Charles D. Ulrich
, Irene Ferber
, Eileen Holicky
, Elizabeth Hadac
, Gary Buell
, Laurence J. Miller

Gastroenterology and Hepatology

Research output: Contribution to journal › Article › peer-review

91 Scopus citations

Abstract

Through binding to cholecystokinin (CCK) A receptors, CCK is an important physiologic regulator of both gallbladder contraction and pancreatic enzyme secretion. In this work, we have used a combination of hybridization screening of a cDNA library and polymerase chain reaction to clone a 2.1 kb cDNA which encodes the human gallbladder CCK_A receptor. Nucleotide sequence analysis revealed an open reading frame encoding a 428 amino acid protein, with seven putative transmembrane domains and a high degree of homology with the rat CCK_A receptor. COS cells transfected with this cDNA clone bound CCK-8 and L-364,718 with high affinities appropriate for the CCK_A receptor, and exhibited a transient increase in intracellular calcium in response to CCK. This should provide an important resource for the analysis of the role of this receptor in human physiology and pathophysiology.

Original language	English (US)
Pages (from-to)	204-211
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	193
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1993.1610
State	Published - May 28 1993

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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AU - Ferber, Irene

AU - Holicky, Eileen

AU - Hadac, Elizabeth

AU - Buell, Gary

AU - Miller, Laurence J.

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AB - Through binding to cholecystokinin (CCK) A receptors, CCK is an important physiologic regulator of both gallbladder contraction and pancreatic enzyme secretion. In this work, we have used a combination of hybridization screening of a cDNA library and polymerase chain reaction to clone a 2.1 kb cDNA which encodes the human gallbladder CCKA receptor. Nucleotide sequence analysis revealed an open reading frame encoding a 428 amino acid protein, with seven putative transmembrane domains and a high degree of homology with the rat CCKA receptor. COS cells transfected with this cDNA clone bound CCK-8 and L-364,718 with high affinities appropriate for the CCKA receptor, and exhibited a transient increase in intracellular calcium in response to CCK. This should provide an important resource for the analysis of the role of this receptor in human physiology and pathophysiology.

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Molecular Cloning and Functional Expression of the Human Gallbladder Cholecystokinin A Receptor

Abstract

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