Abstract
We note that precautions are necessary when ligation-mediated PCR (LMPCR) is applied to the detection of oligonucleotide-directed triple helix formation in vitro and in vivo. Synthetic oligonucleotides applied to cell cultures can persist after chemical treatment and genomic DNA isolation and inhibit a kg step in LMPCR, causing an artifact that simulates a triplex footprint. Residual oligonucleotides apparently form triplexes during LMPCR, blocking ligation of the unidirectional linker in a site-specific manner. We show that careful removal of residual oligonucleotide prior to LMPCR alleviates this problem.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 313-316 |
| Number of pages | 4 |
| Journal | Antisense and Nucleic Acid Drug Development |
| Volume | 9 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1999 |
ASJC Scopus subject areas
- Genetics
- Pharmacology