Induction of L-histidine decarboxylase in a human mast cell line, HMC-1

K. Maeda, H. Taniguchi, I. Ohno, H. Ohtsu, K. Yamauchi, E. Sakurai, Y. Tanno, J. H. Butterfield, T. Watanabe, K. Shirato

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


Histamine is an important mediator in allergic reactions, gastric acid secretions, and neurotransmission in the central nervous system. Basophils and mast cells are the main sources of histamine, which is formed from L- histidine by histidine decarboxylase (HDC). However, the regulatory mechanism of HDC in these cells remains unclear. We examined the regulation of HDC activity and gene expression using a unique human mast cell line, HMC-1, after stimulation with phorbol 12-myristate 13-acetate (PMA) or ionomycin. HDC activity was increased from 52.1 ± 0.4 (mean ± standard deviation) to 154 ± 6.9, or 105.6 ± 6.2 pmol/min/mg protein (n = 3), 4 hours after stimulation with PMA (10 ng/mL) or ionomycin (10-6 M). Although actinomycin D had no effect on this increase, cycloheximide completely inhibited the increase caused by these stimuli. The population of HMC-1 cells containing HDC protein was increased after stimulation with either PMA or ionomycin as evaluated by immunocytochemical analysis with anti-HDC antibody as a marker. HMC-1 constitutively expressed HDC mRNA, and its level was not increased with these stimuli. These results suggest that the increase of HDC activity in HMC-1 induced by PMA or ionomycin is regulated at the translational level.

Original languageEnglish (US)
Pages (from-to)325-331
Number of pages7
JournalExperimental Hematology
Issue number4
StatePublished - 1998


  • Basophils
  • HDC
  • Ionomycin
  • Mast cells
  • PMA

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research


Dive into the research topics of 'Induction of L-histidine decarboxylase in a human mast cell line, HMC-1'. Together they form a unique fingerprint.

Cite this