TY - JOUR
T1 - Increased levels of serum Wisteria floribunda agglutinin-positive Mac-2 binding protein in idiopathic pulmonary fibrosis
AU - Kono, Masato
AU - Nakamura, Yutaro
AU - Oyama, Yoshiyuki
AU - Mori, Kazutaka
AU - Hozumi, Hironao
AU - Karayama, Masato
AU - Hashimoto, Dai
AU - Enomoto, Noriyuki
AU - Fujisawa, Tomoyuki
AU - Inui, Naoki
AU - Yamada, Masaomi
AU - Hamada, Etsuko
AU - Colby, Thomas V.
AU - Maekawa, Masato
AU - Suda, Takafumi
N1 - Funding Information:
This study was partly supported by a grant to the Diffuse Lung Diseases Research Group from the Ministry of Health, Labour and Welfare, Japan . We thank Dr. Shigeki Muto and Mr. Norihisa Maeda (Seirei Center for Health Promotion and Preventive Medicine, Hamamatsu, Japan) for providing the samples from healthy volunteers. We acknowledge Ms. Yayoi Kawabata and Ms. Naomi Suzuki (Department of Preeminent Medical Photonics Education and Research Center, Advanced Research Facilities and Services, Hamamatsu University School of Medicine, Hamamatsu, Japan) for immunohistochemical stains of M2BP.
Publisher Copyright:
© 2016 Elsevier Ltd. All rights reserved.
PY - 2016/6
Y1 - 2016/6
N2 - Background Mac-2 binding protein (M2BP) is a cell-adhesive glycoprotein of the extracellular matrix secreted as a ligand of galectin-3 (Mac-2). Recently, a Wisteria floribunda agglutinin positive-M2BP (WFA+-M2BP) assay developed using a lectin-antibody sandwich immunoassay has shown promise as a new fibrotic marker in liver fibrosis to detect unique fibrosis-related glycoalteration. The aim of this study is to evaluate the utility of serum WFA+-M2BP levels in patients with idiopathic pulmonary fibrosis (IPF). Methods We measured serum WFA+-M2BP levels in 116 patients with IPF and 42 healthy volunteers. We examined the relationship between serum WFA+-M2BP levels and clinical parameters and further investigated the prognostic significance of serum WFA+-M2BP levels in patients with IPF. Results Serum WFA+-M2BP levels were significantly higher in patients with IPF than in healthy controls (1.09 ± 0.89 cutoff index [COI], 0.57 ± 0.24 COI, respectively; P < 0.001). In patients with IPF, a significant positive correlation was found between serum WFA+-M2BP levels and age, KL-6, neutrophils in BAL, reticulation and honeycombing scores in HRCT, and fibrotic foci scores in pathological findings, and a significant negative correlation was found between serum WFA+-M2BP levels and FVC, %DLco and macrophages in BAL. Furthermore, patients with high serum WFA+-M2BP levels had a significantly worse prognosis than those with low levels (log-rank test, P = 0.0209), and a high serum WFA+-M2BP level was a significant prognostic factor in Cox proportional hazards regression analysis. Conclusions Our results suggest that the serum WFA+-M2BP level is a potential biomarker in patients with IPF.
AB - Background Mac-2 binding protein (M2BP) is a cell-adhesive glycoprotein of the extracellular matrix secreted as a ligand of galectin-3 (Mac-2). Recently, a Wisteria floribunda agglutinin positive-M2BP (WFA+-M2BP) assay developed using a lectin-antibody sandwich immunoassay has shown promise as a new fibrotic marker in liver fibrosis to detect unique fibrosis-related glycoalteration. The aim of this study is to evaluate the utility of serum WFA+-M2BP levels in patients with idiopathic pulmonary fibrosis (IPF). Methods We measured serum WFA+-M2BP levels in 116 patients with IPF and 42 healthy volunteers. We examined the relationship between serum WFA+-M2BP levels and clinical parameters and further investigated the prognostic significance of serum WFA+-M2BP levels in patients with IPF. Results Serum WFA+-M2BP levels were significantly higher in patients with IPF than in healthy controls (1.09 ± 0.89 cutoff index [COI], 0.57 ± 0.24 COI, respectively; P < 0.001). In patients with IPF, a significant positive correlation was found between serum WFA+-M2BP levels and age, KL-6, neutrophils in BAL, reticulation and honeycombing scores in HRCT, and fibrotic foci scores in pathological findings, and a significant negative correlation was found between serum WFA+-M2BP levels and FVC, %DLco and macrophages in BAL. Furthermore, patients with high serum WFA+-M2BP levels had a significantly worse prognosis than those with low levels (log-rank test, P = 0.0209), and a high serum WFA+-M2BP level was a significant prognostic factor in Cox proportional hazards regression analysis. Conclusions Our results suggest that the serum WFA+-M2BP level is a potential biomarker in patients with IPF.
KW - Biomarker
KW - Idiopathic pulmonary fibrosis (IPF)
KW - Mac-2 binding protein (M2BP)
KW - Mac-2 binding protein glycosylation isomer (M2BPGi)
KW - Wisteria floribunda agglutinin positive-M2BP (WFA-M2BP)
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U2 - 10.1016/j.rmed.2016.04.013
DO - 10.1016/j.rmed.2016.04.013
M3 - Article
C2 - 27215503
AN - SCOPUS:84965020843
SN - 0954-6111
VL - 115
SP - 46
EP - 52
JO - Respiratory Medicine
JF - Respiratory Medicine
ER -